Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MPINHEPLFDVLSQYDIKV-VSIRNESYKDKKGVWWIQTPD-EYKILKKISNS--EDTFKYILSAAEHLRKN-GVNIPAVYKTKDGKDYVNINGTCYVLYEAVEGKNPSY-----NSPEDFRAIVRELAGFHAASVGFSPPDNTKPKIHLGKWVEQYTEQVEDMNRFYQTELEKSENDRIGKVIIEEFPAFYERAKQAIEGLKGKEYQDWVEKVKSRGGLCHQDFAAGNLLKNPS-GKIFVLDTDSITIDIPARDIRKLLNKIMKKNGKWDLEILRKFIRIYQSENPLSFSEWTVVKFDLMFPHLFLGAMNKFYYKRDKEWSFEKYLKRINEMTALEKTITPVLENFDSIVYEEINQRKD 5IGH Chain:A ((6-257)) -TADTSQLYALAARHGLKLHGPLTVNELGLDYRIVIATVDDGRRWVLRIPRRAEVSAKVEPEARVLAMLKNRLPFAVPDWRVANAELVAYPM-LEDSTAMVIQPGSSTPDWVVPQDSEVFAESFATALAALHAVPISAAVDAGML-IRTPTQARQKVADDV---DRVRREF-----------------VVNDKRLHRWQRWLDDDS------SWPDFSVVVHGDLYVGHVLIDNTERVSGMIDWSEARVDDPAIDMAAHLMVF-------GEEGLAKLLLTYEAAGGR------------------------------------------------------------------------

General information: TITO was launched using: RESULT: Template: 5IGH.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 952 9472 9.95 39.30 target 2D structure prediction score : 0.67 Monomeric hydrophicity matching model chain A : 0.61 3D Compatibility (PKB) : 9.95 2D Compatibility (Sec. Struct. Predict.) : 0.67 1D Compatibility (Hydrophobicity) : 0.61 QMean score : 0.293

(partial model without unconserved sides chains): PDB file : Tito_5IGH.pdb: (Unconserved sides chains are recalculated) : Sequence: align-5igh-query.scw PDB file : Tito_Scwrl_5IGH.pdb: