Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MLAGCDRCRASSPPDPVNESAEFLQLLRQDGVVRTDDARLMPLTGGVSCEIYL-VEDG-AERFVVKRALAKLKVKADWFADICRNRYEWEFIRYVSRFLPAVVPALRHCSATGNYFAMEYLGGEFQNWKQQLLAG-EARPEAAAQAGGLLAEIHRHSTGDAEAKRIFDTTPNFY-QL-RIEAYLL-ATG----VKHPELR-PLFEAEAARLAATRECLVHGDFSPKNILVSPE-----RMVLLDCEVAWYGDPSFDLAFMLNHFFLKTLLHAPREVGMRRMVEAFWAAYQTGRPTPELETRVGRLLVMLLLARVDGKSPVEYLDVGRQDFVRQFARVMLHEEAPSLGVVTNAWFARLSQFTS 3CSV Chain:A ((6-252)) -------------------EDEIRDFLATHGY---ADWNRTP--------RYQRLRSPTGAKAVLMDWSPE---------EGGDTQPFVDLAQYLRNL-DISAPEIYAEEHARGLLLIEDLGD--ALFTEVINNDPAQEMPLYRAAVDLLIHLHDAQTPELAR---LDPETLSEMTRLAFSEYRYAILGDAAEDNRKRFEHRFAQ-ILSAQLEGDMVFVHRDFHAQNLLWLPEREGLARVGVIDFQDAKLGHRAYDLVSLLQDARRDV---------PAQVEAQMIDHYIQATG--------------------------------------------------------------------

General information: TITO was launched using: RESULT: Template: 3CSV.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 916 -25162 -27.47 -112.83 target 2D structure prediction score : 0.60 Monomeric hydrophicity matching model chain A : 0.62 3D Compatibility (PKB) : -27.47 2D Compatibility (Sec. Struct. Predict.) : 0.60 1D Compatibility (Hydrophobicity) : 0.62 QMean score : 0.231

(partial model without unconserved sides chains): PDB file : Tito_3CSV.pdb: (Unconserved sides chains are recalculated) : Sequence: align-3csv-query.scw PDB file : Tito_Scwrl_3CSV.pdb: