Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MQINPALAQAIEADSG-F-ELTQVEFIGAGWFAQAYRFCSQAKHYVVRISKHY---HDFLKDVYAYQHWGQ--QLPIPAILAHGQF---ADGWAYAISPHIAGQTIVSLE-----AAELRQIQPALFKSLQQLHQLKLGPVTGWGSVNSQGHGRYQSWAEEVLDIGNYKFDFDWHDWIKRDDQTGTLLRQGYRLMEQLLSTISTERSLIHRDFGFDNVLCQP--PTIVAVLDWADFGYGDWVYDIAQQSCYGQAEIYLQPWL--KFA-QQHDLIP-DNFEKRLHCYWLRMNLTDLIVSQLRNDWHWQRKIVAELSWLIENPVVVRLNQ 3N4U Chain:A ((3-282)) -TYTFDQVEKAIEQLYPDFTINTIEISGEGNDCIAYEI---NRDFIFKFPKHSRGSTNLFNEVNILKRIHNKLPLPIPEVVFTGMPSETYQ-MSFAGFTKIKGVPLTPLLLNNLPKQSQNQAAKDLARFLSELHSINISGFK---SN------LVLDFREKINEDNKKIKKL-LSRELKG--PQMKKVDDFYRDILENEIYFKYYPCLIHNDFSSDHILFDTEKNTICGIIDFGDAAISDPDNDFISLMEDD----EEYGMEFVSKILNHYKHKDIPTVLEKYRMKEKYWSFEKIIYGKEY---------------------------

General information: TITO was launched using: RESULT: Template: 3N4U.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1116 -11984 -10.74 -46.27 target 2D structure prediction score : 0.54 Monomeric hydrophicity matching model chain A : 0.65 3D Compatibility (PKB) : -10.74 2D Compatibility (Sec. Struct. Predict.) : 0.54 1D Compatibility (Hydrophobicity) : 0.65 QMean score : 0.326

(partial model without unconserved sides chains): PDB file : Tito_3N4U.pdb: (Unconserved sides chains are recalculated) : Sequence: align-3n4u-query.scw PDB file : Tito_Scwrl_3N4U.pdb: