Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MTQAALAPGGFDEFEMHQVLKRGCAAVGLDSSEARL-LRGHTNAVIHLVKEHVVVKIARKGSRIDDVARTVRFVRWLMDA-GFPTVSLHPVDQPVGVG------QHALTFWEYLPQPS--D-----------PVPAAQLAKPLYSLHTLPASPEALP-AHDNLAAIRRSLKSIT-----CLSDEALAFLNGSTEQLESALHVVQFELPEGVIQGDPQHRNALHTA--SGGAVLCDWDTVAIGQPEWDLVTVEVHCRRFGHGSQHYEAFANTYGWD--VTRWSGYQTLAAIRELRMITTNARKVSDAPTSLQEVERRVEGLRCHDRALRWNIL 3N4U Chain:A ((3-298)) ---------TYTFDQVEKAIEQLYPDF--TINTIEISGEGNDCIAYEI-NRDFIFKFPKHSRGSTNLFNEVNILKRIHNKLPLPI------PEVVFTGMPSETYQMSFAGFTKIKGVPLTPLLLNNLPKQSQNQAAKDLARFLSELHSINISGFKSNLVLDFREKINEDNKKIKKLLSRELKGPQMKKVDDFYRDILENEI--YFKYYPCLIHNDFSSDHILFDTEKNTICGIIDFGDAAISDPDNDFISLMEDDEEY--GMEFVSKILNHYKHKDIPTVLEKYRMKEKYWSFEKIIYGKEYGYM--DWYEEGLNEIRSI------------

General information: TITO was launched using: RESULT: Template: 3N4U.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1160 1775 1.53 6.70 target 2D structure prediction score : 0.65 Monomeric hydrophicity matching model chain A : 0.65 3D Compatibility (PKB) : 1.53 2D Compatibility (Sec. Struct. Predict.) : 0.65 1D Compatibility (Hydrophobicity) : 0.65 QMean score : 0.375

(partial model without unconserved sides chains): PDB file : Tito_3N4U.pdb: (Unconserved sides chains are recalculated) : Sequence: align-3n4u-query.scw PDB file : Tito_Scwrl_3N4U.pdb: