Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MSAPTEEMISVQKLVERLNAEYAFEVDYIGADLGGFSSQNNQLTLKDKRRMVLKRCDQSQWDPRNVDIATILAHHSS-LIYAPLATVGGSSTFKFDGWVYAVFPYVDGEKLHQHSLDAETLKSAA-RALYAFHHIA----------------HFAGSHAAQRPN-VSDFE-REVTSVLEPTLSLSTDHADIIIRSIEAKRHILHSFSSDLSSINVGASSVIHGDFHNENILYRSDHVVAILDFEMLHWGNVEDDIIS-FIALGCCNNGFSEENLRM-ANIFVKEYLSLHGENLDLHTALLRF-IHL-KSSSLFLQKMLIYTGNMLYAQIIERDNKLFPSIMKKHREISKTLSSL- 2Q83 Chain:A ((11-346)) --LSAEDAKKLTELAENVLQGWDVQAEKIDV-----IQALVWKVHTDSGAVCLKRIHRPEKKALFSIFAQDYLAKKGMNVPGILPNKKGSLYSKHGSFLFVVYDWIEGRPF---ELTVKQDLEFIMKGLADFHTASVGYQPPNGVPIFTKLGRWPNHYTKRCKQMETWKLMAEAEKEDPFSQLYLQEIDGFIEDGLRIKDRLLQSTYVPWTEQLKKSPNLCHQDYGTGNTLLGENEQIWVIDLDTVSFDLPIRDLRKMIIPLLDTTGVWDDETFNVMLNAYESRA--------PLTEEQKQVMFIDMLFPYELYDVIREKYVRKS-----ALPKEELESAFEYERIKANALRQLI

General information: TITO was launched using: RESULT: Template: 2Q83.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1290 -30961 -24.00 -100.85 target 2D structure prediction score : 0.53 Monomeric hydrophicity matching model chain A : 0.61 3D Compatibility (PKB) : -24.00 2D Compatibility (Sec. Struct. Predict.) : 0.53 1D Compatibility (Hydrophobicity) : 0.61 QMean score : 0.365

(partial model without unconserved sides chains): PDB file : Tito_2Q83.pdb: (Unconserved sides chains are recalculated) : Sequence: align-2q83-query.scw PDB file : Tito_Scwrl_2Q83.pdb: