Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MSVYTELSEQDVMHLLADYDLG-VYVRHQGISAGVENTNYFVSTD-QHELVLTVFEKH-SADELPFFLQLGEHLHA-RHCKVPQPFRDRDGQFLQIVKGKPAVFIERLTGQHVQA--------------SPACALKIAQALADIHNATLSFQTDQRH-S----HN-RGWIERQASRVLPSLSAPDQQLM-NAALAIIRAI-PDDLPEGVIHADLFHDNALFDGNEVAGIIDWYFAGRDSYALDIAITMNDWCLDTQHQVDPEQCAAFIERYHQRRSLSSAERNALPKLQVQSALRFWISRLLAQAEHGESNDSITVKDPIPMREQCRQLLTRVREPGNEHR 5UXD Chain:A ((3-256)) ----MPEDLDALLDLAARHGLDLDGGTLRTEEIGLDFRVAFARAHDGGDWVLRLPRRPDVLERAAVEGRLLAMLAPHLDVAVPDWRIST----------SELIAYPLLPGSPGLTVAADGEVSWHVDMASTVYARSLGSVVAQLHAVDAEAA-AATGIEVRSPAQVRGAWRQDLARVGAEF--EIAPALRERWEAWLADDGCWPGHSVLTHGELYPAHTLVEDERITAVLDWTTAAVGDPAKDLMFHQVSA--------PSAIFEVALQAYAEGGGRPW--------------------------------------------------------------

General information: TITO was launched using: RESULT: Template: 5UXD.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 903 -23308 -25.81 -102.23 target 2D structure prediction score : 0.65 Monomeric hydrophicity matching model chain A : 0.63 3D Compatibility (PKB) : -25.81 2D Compatibility (Sec. Struct. Predict.) : 0.65 1D Compatibility (Hydrophobicity) : 0.63 QMean score : 0.271

(partial model without unconserved sides chains): PDB file : Tito_5UXD.pdb: (Unconserved sides chains are recalculated) : Sequence: align-5uxd-query.scw PDB file : Tito_Scwrl_5UXD.pdb: