Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MTQAALAPGGFDEFEMHQVLKRGCAAVGLDS-SEARLLRGHTNAVIHLVK--EHVVVKIARKGS-RIDDVARTVRFVRWLMD-AGFPTVSLHPV--DQPV-GV----GQHALTFWEYLPQPS------DPVPAAQLAKPLYSLHTLPASPEALPAHD-NLAAIRRSLK---SITC------LSDEALAFLNGSTEQLESALHVV-QFELPEGVIQGDPQHRNALHTAS---GGAVLCDWDTVAIGQPEWDLVTVEVHCRRFGHGSQHYEAFANTYGW----DVTRWSGYQTLAAIRELRMITTNARKVSDAPTSLQEVERRVEGLRCHDRALRWNIL 6EF6 Chain:A ((30-324)) ------------------IAQQALTHYDVSDNASLRLLNLSENATYLVEDGEHQSILRVHRQDYHQPHEIESELDWLAALRTDSDVTVPTVVPARDGRRVVTVDPADVPRHVVHFEMVGGAEPDEESLTLDDFQTLGRITASLHEHSQRWTRPAGFGRFSWDWEHCLGDTPRWGRWQDAEGVGASETALLTRAQDLLHRKLEEYGSGPDRYGLIHADLRLANLLVDSSTPQRTITVIDFDDCGFGWYFYDFGTAVSFIEHDPRLGEWQESWVAGYRSRRELPAADEAMLPSFVFLRRLLLLAWMGSHTHS---------------------------

General information: TITO was launched using: RESULT: Template: 6EF6.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1134 -4506 -3.97 -17.60 target 2D structure prediction score : 0.64 Monomeric hydrophicity matching model chain A : 0.69 3D Compatibility (PKB) : -3.97 2D Compatibility (Sec. Struct. Predict.) : 0.64 1D Compatibility (Hydrophobicity) : 0.69 QMean score : 0.281

(partial model without unconserved sides chains): PDB file : Tito_6EF6.pdb: (Unconserved sides chains are recalculated) : Sequence: align-6ef6-query.scw PDB file : Tito_Scwrl_6EF6.pdb: