Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MDDFAEWVRVAFGLGEGPLVRTVGGRGADGDVWHLRVRDREYAVKRPFRAF-DPAAVRHEAALLEHLTS-GGVEVPTHVASPEGQLVVEVPD--HLGGGNARVSHWVTGTSVTDR--TSEVAEQLGTLLAQLHRAAPATWDRPRSWHTAMVPPDEWAELVR--RSSGQPW-----HQALVARHD-DLTAYAEVVDRAGPGTGPFVLGHCDLHPDNALAAPD---GSLRALDWEDCGPVDPTRELAKTLVQWHVLGESVDDDAITKTVAAYRDAGGPGLLA--DTPDFAMVLCSETNFLANQVRLALDVSAPGERRDQARGEITEALADYLPSIAALERVLSVTRPG 6EF6 Chain:A ((28-314)) -VTIAQQALTHYDVSDN-ASLRLL-NLSENATYLVEDGEHQSILRVHRQDYHQPHEIESELDWLAALRTDSDVTVPTVVPARDGRRVVTVDPAD--VPRHVVHFEMVGGAEPDEESLTLDDFQTLGRITASLHEHSQRW-T-RPAGFGRFSWD--WEHCLGDTPRWGRWQDAEGVGASETALLTRAQDLLHRKLEEYGSGPDRYGLIHADLRLANLLVDSSTPQRTITVIDFDDCGFGWYFYDFGTAVSFIEHDP--RLGEWQESWVAGYRSRRELPAADEAMLPSFVFLRRLLL---------------------------------------------------

General information: TITO was launched using: RESULT: Template: 6EF6.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1200 -14419 -12.02 -54.41 target 2D structure prediction score : 0.65 Monomeric hydrophicity matching model chain A : 0.65 3D Compatibility (PKB) : -12.02 2D Compatibility (Sec. Struct. Predict.) : 0.65 1D Compatibility (Hydrophobicity) : 0.65 QMean score : 0.295

(partial model without unconserved sides chains): PDB file : Tito_6EF6.pdb: (Unconserved sides chains are recalculated) : Sequence: align-6ef6-query.scw PDB file : Tito_Scwrl_6EF6.pdb: