Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MRSEALEQFVVEEAGVGAPR-------------GASWSRVDEGQ-------GA--NRVWRVRW--------GPGSSVIVKRCGPGRAFVQERRAATRWAPAVADASRGLGHARVLAASQALRCLVFVDAGRGEEQVALSPAAFACAGRYLARLHALPVDERDPVPLVEALARREARWLEG--APELRA-VASSLGPGALA---------------DADWGPRVPCHRDFQPDNWLWDGRTLTVVDFEHARPDARALDLAKLWT--RLDGDVDARANA--------------AFWSAYL-----GPEPCSPSFLAQLRAGL-VLHGLASVAWG--------RSHDDEAFVAEGERAVALARSGREPPNPRAGTPLASL- 1NW1 Chain:A ((32-426)) GMKELLSTMDLDTDANTIPELKERAHMLCARFLGGAWKTVPLEHLRISRIKGGMSNMLFLCRLSEVYPPIRNEPNKVLLRVYFNPETESHLVAESVI---FTLLSERHLG-PKLYGIFSGGRLEEYIPSRPLSCHEISLAHMSTKIAKRVAKVHQLEVPIWKEPDYLCEALQRWLKQLTGTVDAEHRFDLPEECGVSSVNCLDLARELEFLRAHISLSKSPVTFCHNDLQEGNILLPKR-LVLIDFEYASYNYRAFDFANHFIEWTIDYDIDEAPFYKIQTENFPENDQMLEFFLNYLREQGNTRENELYKKSEDLVQETLPFVPVSHFFWGVWGLLQVELSPVGFGFADYGRDRLSLYFKHKQ--------LLKNLA

General information: TITO was launched using: RESULT: Template: 1NW1.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1191 8765 7.36 30.65 target 2D structure prediction score : 0.74 Monomeric hydrophicity matching model chain A : 0.64 3D Compatibility (PKB) : 7.36 2D Compatibility (Sec. Struct. Predict.) : 0.74 1D Compatibility (Hydrophobicity) : 0.64 QMean score : 0.374

(partial model without unconserved sides chains): PDB file : Tito_1NW1.pdb: (Unconserved sides chains are recalculated) : Sequence: align-1nw1-query.scw PDB file : Tito_Scwrl_1NW1.pdb: