Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MMKKVPEFVVQFSPESFSLTPVPGGLSNHNYRLQFQQH-----GLNNRYFVRQFSATYLGMDNDVELEYAAQIQAANIGLAPNVIVKHKQGMICDWVTGEHWDQTAQVRDENIEKLAQLVAMLHQQPMPSHHL--DMVERLQHYYQTLNTE---------FKT----VQLAQQLTLVIDLIEQH-LPANRLGFCHHDMNPLNFIEDEQ------AKLYLLDWEFAAAGHCDFDIATLFQTFEW------------------QDAQQALFIRYYNQYYPA-------------AKVTFEQLDVMAVVVEMMTLLWCIVMYQQD-KDATYLRLWQQSEYAITDKINRLSKELQWDQ 5FTG Chain:A ((30-378)) --------------DEFHISVIRGGLSNMLFQCSLPDTTATLGDEPRKVLLRLYG-------EAMVLESVMFAILAERSLGPKLYGIFPQGRLEQFIPSRRLDTEELSLPDISAEIAEKMATFHGMKMPFNKEPKWLFGTMEKYLKEVLRIKFTEESRIKKLHKLLSYNLPLELEN---LRSLLESTPSPVVFCHNDCQEGNILLLEGRENSEKQKLMLIDFEYSSYNYRGFDIGNHFCEWMYDYSYEKYPFFRANIRKYPTKKQQLHFISSYLPAFQNDFENLSTEEKSIIKEEMLLEVNRFALASHFLWGLWSIVQAKISSIEFGYMDYAQARFDAYFHQKRKLGV------

General information: TITO was launched using: RESULT: Template: 5FTG.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 929 -23412 -25.20 -88.35 target 2D structure prediction score : 0.70 Monomeric hydrophicity matching model chain A : 0.65 3D Compatibility (PKB) : -25.20 2D Compatibility (Sec. Struct. Predict.) : 0.70 1D Compatibility (Hydrophobicity) : 0.65 QMean score : 0.387

(partial model without unconserved sides chains): PDB file : Tito_5FTG.pdb: (Unconserved sides chains are recalculated) : Sequence: align-5ftg-query.scw PDB file : Tito_Scwrl_5FTG.pdb: