Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MDDFAEWVRVAFGLGEGPLVRTVGGRGADGDVWHL-RVRDR-EYAVKRPFRAFDPAAVRHEAALLEHLTSGGVE-VPTHVASPEGQLVVEVPDHLGGGNARVSHWVTGTSVTDRTSEVAEQLGTLLAQLHRAAPATWDRPRSWHTAMVPPDEWAELVRRSSGQ------------PWHQALVARHD-DLTAYAEVV---------DRAGPGTGPFVLGHCDLHPDNALAAPDGSLRALDWEDCGPVDPTRELAKTLVQWHVLGESVDDDAITKTVAAYRDAGGPGLLA--DTPDFAMVLCSETNFLANQVRLALDVSAPGERRDQARGEITEALADYLPSIAALERVLSVTRPG 4PDY Chain:A ((24-302)) ----PQAVVSKYDLAI--QQ---RHA---DGNIEVWTDSKGRRYAAKRSSIA--PAHCRIMVQCLRHAQEQGFTKFARFVTTSSNAPYVRH----GDFTYYVTEWVSGQPANFGLPEHVAQTAYTLAQFHEATRSF-R-TDWK---DDVFGLFQARWR-DLRQMWLGADRKREKDAFDQLLLSMRDELHRDAAESLALFEDRDVIAYLEAERSSGGWCHLDVIPSNCLYTPQHQVVLIDFELARPAPRALDMAHLLRRSLERG-NWDGHLAYACFLHFDAVRNIPKSEYRAVEAILRFP-------------------------------------------------------

General information: TITO was launched using: RESULT: Template: 4PDY.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 986 7921 8.03 32.07 target 2D structure prediction score : 0.71 Monomeric hydrophicity matching model chain A : 0.64 3D Compatibility (PKB) : 8.03 2D Compatibility (Sec. Struct. Predict.) : 0.71 1D Compatibility (Hydrophobicity) : 0.64 QMean score : 0.279

(partial model without unconserved sides chains): PDB file : Tito_4PDY.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4pdy-query.scw PDB file : Tito_Scwrl_4PDY.pdb: