Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MMKKVPEFVVQFSPESFSLTPVPGGLSNHNYRLQFQQH-----GLNNRYFVRQFSATYLGM-DNDVELEYAAQIQAANIGLAPNVIVKHKQGMICDWVTGEHWDQTAQVRDENIEKLAQLVAMLHQQPMPSHHL--DMVERLQHYYQTLNTE---------FKT----VQLAQQLTLVIDLIEQH-LPANRLGFCHHDMNPLNFIEDEQ------AKLYLLDWEFAAAGHCDFDIATLFQTFEW------------------QDAQQALFIRYYNQYYPA-------------AKVTFEQLDVMAVVVEMMTLLWCIVMYQQD-KDATYLRLWQQSEYAITDKINRLSKELQWDQ 5FUT Chain:A ((30-378)) --------------DEFHISVIRGGLSNMLFQCSLPDTTATLGDEPRKVLLRLYG------GAEAMVLESVMFAILAERSLGPKLYGIFPQGRLEQFIPSRRLDTEELSLPDISAEIAEKMATFHGMKMPFNKEPKWLFGTMEKYLKEVLRIKFTEESRIKKLHKLLSYNLPLELEN---LRSLLESTPSPVVFCHNDCQEGNILLLEGRENSEKQKLMLIDFEYSSYNYRGFDIGNHFCEWMYDYSYEKYPFFRANIRKYPTKKQQLHFISSYLPAFQNDFENLSTEEKSIIKEEMLLEVNRFALASHFLWGLWSIVQAKISSIEFGYMDYAQARFDAYFHQKRKLGV------

General information: TITO was launched using: RESULT: Template: 5FUT.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 931 -2094 -2.25 -7.87 target 2D structure prediction score : 0.69 Monomeric hydrophicity matching model chain A : 0.65 3D Compatibility (PKB) : -2.25 2D Compatibility (Sec. Struct. Predict.) : 0.69 1D Compatibility (Hydrophobicity) : 0.65 QMean score : 0.399

(partial model without unconserved sides chains): PDB file : Tito_5FUT.pdb: (Unconserved sides chains are recalculated) : Sequence: align-5fut-query.scw PDB file : Tito_Scwrl_5FUT.pdb: