Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MLIEDKIVEIATRVFQV-NE---IDVRVDSRFKGGMSNYTYLVYVKE---------QPYVIRIIGDGGEVL--VKPSIEKKHIQQAKALNLNSELVYFDSKTGVKVSKYVEGTPLSVS--MEETDYPLVANALKTLHQAKLPGE-DY-GLKERLRRYEKLLKKEPS------TTYY-TLKMQWLKLY---DAYF--SKLPKVLCHGDAQRSNLVKSG-------DKIYLLDWEFSGLNDPYYDIASFGN-ID-----------------F---KDAEKLLGYYLERTP--------------SAFELAHIRFYRMYQVLQWHIVATYKHEINLSEKLHLDFEMIAGKYLSLAGHFLEQLKEIEQFK 5FUT Chain:A ((11-376)) -----RAYLWCKEFLPGAWRGLREDEFHISVIRGGLSNMLFQCSLPDTTATLGDEPRKVLLRLYG------GAEAMVLESVMFAILAERSLGPKLYGIF--PQGRLEQFIPSRRLDTEELSLPDISAEIAEKMATFHGMKMPFNKEPKWLFGTMEKYLKEVLRIKFTEESRIKKLHKLLSYNLPLELENLRSLLESTPSPVVFCHNDCQEGNILLLEGRENSEKQKLMLIDFEYSSYNYRGFDIGNHFCEWMYDYSYEKYPFFRANIRKYPTKKQQLHFISSYLPAFQNDFENLSTEEKSIIKEEMLLEVNRFALASHFLWGLWSIVQAKISS---IEFGYMDYAQARFDAYFHQKRKL-------

General information: TITO was launched using: RESULT: Template: 5FUT.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1027 -12639 -12.31 -46.81 target 2D structure prediction score : 0.62 Monomeric hydrophicity matching model chain A : 0.67 3D Compatibility (PKB) : -12.31 2D Compatibility (Sec. Struct. Predict.) : 0.62 1D Compatibility (Hydrophobicity) : 0.67 QMean score : 0.430

(partial model without unconserved sides chains): PDB file : Tito_5FUT.pdb: (Unconserved sides chains are recalculated) : Sequence: align-5fut-query.scw PDB file : Tito_Scwrl_5FUT.pdb: