Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MMKKVPEFVVQFSP--ESFSLTPVPGGLSNHNYRLQFQQHGLNNRYFVRQFSATYLGMDNDVELEYAAQIQAANIGLAPNVIVKHKQ--GMICDWVTGE-HWDQTAQV-RDENIEKLAQLVAMLHQQPMPSH-HLDMVERLQHYYQTLNTE--FKTVQLAQQLTLVIDLIEQH-LPANRLGFCHHDMNPLNFIEDEQAKLYLLDWEFAAAGHCDFDIATLFQTFEWQDAQQALFIRYYNQYYPAAKVTFEQLDVMAVVVEMMTLLWCIVMYQQDK-DATYLRLWQQSEYAITDKINRLSKELQWDQ 3DXQ Chain:B ((4-286)) --DEARAKLAAIPMLAGYTGPLERLGGLTNLVFRA--------GDLCLRIP---------NRANEAVAAREAAKAGVSPEVLHVDPATGVMVTRYIAGAQTMSPEKFKTRPGSPARAGEAFRKLHGSGAVFPFRFELFAMIDDYLKVLSTKNVTLPAGYHDVVREAGGVRSALAAHPLPLAACHCDPLCENFLDTG-ERMWIVDWEYSGMNDPLWDLGDLSVEGKFNANQDEELMRAYFGGEA-RPAERGRVVIYKAMCDLLWTLWGLIQLANDNPVDDFRAYADGRFARCKALME----------

General information: TITO was launched using: RESULT: Template: 3DXQ.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain B - contact count / total energy / energy per contact / energy per residue : 1246 5386 4.32 20.40 target 2D structure prediction score : 0.67 Monomeric hydrophicity matching model chain B : 0.66 3D Compatibility (PKB) : 4.32 2D Compatibility (Sec. Struct. Predict.) : 0.67 1D Compatibility (Hydrophobicity) : 0.66 QMean score : 0.319

(partial model without unconserved sides chains): PDB file : Tito_3DXQ.pdb: (Unconserved sides chains are recalculated) : Sequence: align-3dxq-query.scw PDB file : Tito_Scwrl_3DXQ.pdb: