Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MTRDALDRAAVTAFLVARHGDAVCDSAIEIRPLPGGLCSEVALVTARLRGAAGARRVTLVAKL-V----HGTAAREAAVYRRLSRSVGRRFSPELLGAERLGRGRWLLYLERIEPAHAWPWAD-------------SGHTARVLERLARLHEETPQGAALPAWDYEAENLAMA-RDTLSLAERLPRG-EVPALARALPALRRVVSALPAMRRQLLAFQPLGRAVIHGDVHPGNVMVRRRDGVVEPVLLDWARARMGSPLEDVSSWLQALGYWEPEARRRHDTLLAGYLSARGLPPALTRPLRDVYWFASASNALAGALGHHLAMACGASAPGVLAAAARNAADWLRVIRRADACWS 5UXD Chain:A ((3-255)) ---MPEDLDALLDLAARHGLD---LDGGTLRTEEIGLDFRVAFARAHD---G----GDWVLRLPRRPDVLERAAVEGRLLAMLAPH-LDVAVPDWRIST-----SELIAYPLLP-GSPGLTVAADGEVSWHVDMASTVYARSLGSVVAQLHAVDAEAAAATGIEV-RSP-AQVRGAWRQDLARVGAEFEIAPA-----LRERWEAWLADDG-----CWPGHSVLTHGELYPAHTLVED-E--RITAVLDWTTAAVGDPAKDLMFHQV--S-APSAIFEV---ALQAYAEGGGRP--------------------------------------------------------------

General information: TITO was launched using: RESULT: Template: 5UXD.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 934 -25859 -27.69 -110.98 target 2D structure prediction score : 0.61 Monomeric hydrophicity matching model chain A : 0.61 3D Compatibility (PKB) : -27.69 2D Compatibility (Sec. Struct. Predict.) : 0.61 1D Compatibility (Hydrophobicity) : 0.61 QMean score : 0.167

(partial model without unconserved sides chains): PDB file : Tito_5UXD.pdb: (Unconserved sides chains are recalculated) : Sequence: align-5uxd-query.scw PDB file : Tito_Scwrl_5UXD.pdb: