Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MTRDALDRAAVTAFLVARHGDAVCDSAIEIRPLPGGLCSEVAL-VTARLRGAAGARRVTLVAKL-V---HGTAAREAAVYRRLSRSVGRRFSPELLGAERLGRGRWLLYLERIEPAHAWPWAD-------SGHTARVLERLARLHEETPQGAALPAWDYEAENLAMA-RDTLSLAERLPRGEVPALARALPALRRVVSALPAMRRQLLAFQPLGRAVIHGDVHPGNVMVRRRDG--VVEPVLLDWARARMGSPLEDVSSWLQALGY-WEPEARRRHDTLLAGYLSARGLPPALTRPLRDVYWFASASNALAGALGHHLAMACGASAPGVLAAAARNAADWLRVIRRADACWS 3CSV Chain:A ((4-256)) -----SREDEIRDFLAT-HG----YADWNRTP--------RYQRLRSPT---G----AKAVLMDWSPEEGGDTQPFVDLAQYLRNL--DISAPEIYAEEH---ARGLLLIEDLG-DALFTEVINNDPAQEMPLYRAAVDLLIHLHDAQTPELA--RLDP-ETLSEMTRLAFSEYRYA----ILGDAAED--NRKRFEHRFAQILSA---QLEGDMVFVHRDFHAQNLLWLP-EREGLARVGVIDFQDAKLGHRAYDLVSLLQDARRDVPAQVEAQ---MIDHYIQATGVDES------------------------------------------------------------

General information: TITO was launched using: RESULT: Template: 3CSV.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 948 773 0.82 3.38 target 2D structure prediction score : 0.65 Monomeric hydrophicity matching model chain A : 0.63 3D Compatibility (PKB) : 0.82 2D Compatibility (Sec. Struct. Predict.) : 0.65 1D Compatibility (Hydrophobicity) : 0.63 QMean score : 0.222

(partial model without unconserved sides chains): PDB file : Tito_3CSV.pdb: (Unconserved sides chains are recalculated) : Sequence: align-3csv-query.scw PDB file : Tito_Scwrl_3CSV.pdb: