Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MTQAALAPGGFDEFEMHQVLKRGCAAVGLDSSEARL-LRGHTNAVIHLVK-----------EHVVVKIARKGSRIDDVARTVRFVRWLMDAGFPTVSLHPVDQPVGVGQHALTFWEYLPQPS-------DPVPAAQLAKPLYSLHTLPASPEALPA--HDNLAAIRRSLKSIT-C-----LSDE------ALAFLNGSTEQLESALHVVQFELPEGVIQGDPQHRNALHTASGGAVLCDWDTVAIGQPEWDLVTVEVHCRR-FG--------------HGSQHYEAFANTYGWDV-------------TRWSGYQTLAAIRELRMITTNARKVSDAP---TSLQEVERRVEGLRCHDRALRWNIL 1NW1 Chain:B ((76-414)) -------------------------------LRISRIKGGMSNMLFLCRLSEVYPPIRNEPNKVLLRVYFNPETESHLVAESVIFTLLSERHLG-------PKLYGIF-SGGRLEEYIPSRPLSCHEISLAHMSTKIAKRVAKVHQLEVPIWKEPDYLCEALQRWLKQLTGTVDAEHRFDLPEECGVSSVNCLDLARELEFLRAHIS--LSKSPVTFCHNDLQEGNILLPK--RLVLIDFEYASYNYRAFDFANHFIEWTIDYDIDEAPFYKIQTENFPENDQMLEFFLNYLREQGNTRENELYKKSEDLVQETLPFVPVSHFFWGVWGLLQVELSPVGFGFADYGRDRLSL-------------

General information: TITO was launched using: RESULT: Template: 1NW1.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain B - contact count / total energy / energy per contact / energy per residue : 954 -19050 -19.97 -77.76 target 2D structure prediction score : 0.69 Monomeric hydrophicity matching model chain B : 0.61 3D Compatibility (PKB) : -19.97 2D Compatibility (Sec. Struct. Predict.) : 0.69 1D Compatibility (Hydrophobicity) : 0.61 QMean score : 0.209

(partial model without unconserved sides chains): PDB file : Tito_1NW1.pdb: (Unconserved sides chains are recalculated) : Sequence: align-1nw1-query.scw PDB file : Tito_Scwrl_1NW1.pdb: