Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MTGHGMAGDDWQYRKTMPQNILRPTVEAALPHV------AGAEWEPLAGGRTNPVFKIN-A---------DAPLVCKLSCLSGETPLFGNRALDEAAILKHLEGSGLAPSLTDVLAVPEGEILIYSFVEGDILTQT-------IPAAVRILRQVHQAEIPDNLPRLDGSSAAIIQKTKAILETLP------------VQ---------K-AM-AISFAEPKVELPDTLVPRLIHGDPVPANFIQSKGKLTLIDWQCPAIGDPCEDLAIALSPAMNAVYG----------DGPLTPQQMKECLNAYGD-RT-------------VSDRYRKLAPLFHWRMAAYCAWKVEQGD--KDYAAGVKLELAALK 1NW1 Chain:B ((53-412)) ------------------KERAHMLCARFLGGAWKTVPLEHLRISRIKGGMSNMLFLCRLSEVYPPIRNEPNKVLLRVYFN--P--ETESHLVAESVIFTLLSERHLGPKLYGIFS---G-GRLEEYIPSRPLSCHEISLAHMSTKIAKRVAKVHQLEVPI-WKEPDY----LCEALQRWLKQLTGTVDAEHRFDLPEECGVSSVNCLDLARELEFLRA-HIS-LSKSPVTFCHNDLQEGNILLPK-RLVLIDFEYASYNYRAFDFANHFIEWTIDYDIDEAPFYKIQTENFPENDQMLEFFLNYLREQGNTRENELYKKSEDLVQETLPFVPVSHFFWGVWGLLQVELSPVGFGFADYGRDRL----

General information: TITO was launched using: RESULT: Template: 1NW1.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain B - contact count / total energy / energy per contact / energy per residue : 1062 -20719 -19.51 -80.30 target 2D structure prediction score : 0.62 Monomeric hydrophicity matching model chain B : 0.64 3D Compatibility (PKB) : -19.51 2D Compatibility (Sec. Struct. Predict.) : 0.62 1D Compatibility (Hydrophobicity) : 0.64 QMean score : 0.358

(partial model without unconserved sides chains): PDB file : Tito_1NW1.pdb: (Unconserved sides chains are recalculated) : Sequence: align-1nw1-query.scw PDB file : Tito_Scwrl_1NW1.pdb: