Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MDDFAEWVRVAFGLGEGPLVRTVGGRGADGDVWHLRVRD-REYAVKRPFRAFDPAAVRHEAALLEHLTSG-GVEVPTHVASPEGQLVVEVPDHLGGGNARVSHWVTGTSVTD-----RTSEVAEQLGTLLAQLHRAAPATWDRPRSWHTA--M-VPPDEWAELVRRSSGQPWHQALVARHDDLTAYAEVVDRAGPGTGPFVLGHCDLHPDNALAAPDG-SLRALDWEDCGPVDPTRELAKTLVQWHVLGESVDDDAITKTVAAYRDAGGPGLLADTPDFAMVLCSETNFLANQVRLALDVSAPGERRDQARGEITEALADYLPSIAALERVLSVTRPG 5IGI Chain:A ((9-258)) -TSQLYALAARHGLKLH-GPLTVNELGLDYRIVIATVDDGRRWVLRIPRRAEVSAKVEPEARVLAMLKNRLPFAVPDWRVANAELVAYP-----MLEDSTA-VIQPGSSTPDWVVPQDSEVFAESFATALAALHAVPISA-A-VDAGMLIRTPTQARQKVADDVD-RVRREFV-VNDK---RLHRWQRWLDDDSSWPDFSVVVHGDLYVGHVLIDNTERVSGMIDWSEARVDDPAIDMAAHLMVF-------GEEGLAKLLLTYEAAGGRV-------------------------------------------------------------------

General information: TITO was launched using: RESULT: Template: 5IGI.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 997 -5938 -5.96 -24.95 target 2D structure prediction score : 0.70 Monomeric hydrophicity matching model chain A : 0.64 3D Compatibility (PKB) : -5.96 2D Compatibility (Sec. Struct. Predict.) : 0.70 1D Compatibility (Hydrophobicity) : 0.64 QMean score : 0.202

(partial model without unconserved sides chains): PDB file : Tito_5IGI.pdb: (Unconserved sides chains are recalculated) : Sequence: align-5igi-query.scw PDB file : Tito_Scwrl_5IGI.pdb: