Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MISNTPSFPASWNIEAA---KRIADTPAGIVYELTRKDGSLAIAKVLKKKV----LKDSLRGADFIAWRAGIGCVELLDQSDN---------ILLMEHA-GTETLRDVLFRDGNDDATTEIAAEVLLRYHQPSEQSPPS-SLL--TLPLYFESLFRKAEQDRS--DGVDSPFVEAARLAQTLIDQQRDIKPLHGDLHHENIMHSD--RGWIIIDPA-GLIGDAALDVANMFSNPLDRFDLTRSEARIASMAAIFSRALQRDE----RLLLQYAFAYGCLSATWHEEDGNAEERDNELAVAATIKAVLKHF 5IGH Chain:A ((11-299)) ---QLYALAARHGLKLHGPLTVNELGLDYRIVIATVDDGRRWVLRIPRRAEVSAKVEPEARVLAMLKNRLPFAVPDWRVANAELVAYPMLEDSTAMVIQPGS-STPDWV-VPQDSEVFAESFATALAALHAVPISAAVDAGMLIRTPTQARQKVADDVDRVRREFVVNDKRLHRWQRWLDDDSSWPDFSVVVHGDLYVGHVLIDNTERVSGMIDWSEARVDDPAIDMAAHLMVFGEE--------GLAKLLLTYEAAGGRVWPRLAHHIAERLAFGAVTYALFALDSG----NEEYLAAAKAQLAA----

General information: TITO was launched using: RESULT: Template: 5IGH.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1135 18855 16.61 72.52 target 2D structure prediction score : 0.56 Monomeric hydrophicity matching model chain A : 0.62 3D Compatibility (PKB) : 16.61 2D Compatibility (Sec. Struct. Predict.) : 0.56 1D Compatibility (Hydrophobicity) : 0.62 QMean score : 0.245

(partial model without unconserved sides chains): PDB file : Tito_5IGH.pdb: (Unconserved sides chains are recalculated) : Sequence: align-5igh-query.scw PDB file : Tito_Scwrl_5IGH.pdb: