Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MAKPELIELATSCFGL-------TVDEQLPIGESFASEVLRFRSG---------RQCYVLKRPFSL---EKAEREYFWLKRLSH-CPFVPDALEITKANDHGLILMTSLEGMPLKSFHQLSHRDLSKLGHDLRALHTVSADSFDGHKSWRELLLSNTDRYIDKIIGPA--------RKT-----AE-MAYA-RFHEGIDEI-PDSYLPTATHFDFRDGNILADESGYTGIIDFESMRGGHASMDFFKLLTHPSDM-----------------NDIELTALLAGYGSADWLESM----------AQLKHLVACYAVYHGLAGLAWCSQREQT---STEFYQRCAGFLEQGR 1NW1 Chain:B ((55-412)) ----RAHMLCARFLGGAWKTVPLEHLRISRIKGGMSNMLFLCRLSEVYPPIRNEPNKVLLRVYFNPETESHLVAESVIFTLLSERHLG-PKLYGIF----SGGRLEEYIPSRPLSCHEISLAHMSTKIAKRVAKVHQLEVPIWKEPDY----LCEALQRWLKQLTGTVDAEHRFDLPEECGVSSVNCLDLARELEFLRAHISLSKSPVTFCHNDLQEGNILLPK--RLVLIDFEYASYNYRAFDFANHFIEWTIDYDIDEAPFYKIQTENFPENDQMLEFFLNYLRE---QGNTRENELYKKSEDLVQETLPFVPVSHFFWGVWGLLQVELSPVGFGFADYGRDRL----

General information: TITO was launched using: RESULT: Template: 1NW1.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain B - contact count / total energy / energy per contact / energy per residue : 1077 -34065 -31.63 -130.02 target 2D structure prediction score : 0.64 Monomeric hydrophicity matching model chain B : 0.65 3D Compatibility (PKB) : -31.63 2D Compatibility (Sec. Struct. Predict.) : 0.64 1D Compatibility (Hydrophobicity) : 0.65 QMean score : 0.302

(partial model without unconserved sides chains): PDB file : Tito_1NW1.pdb: (Unconserved sides chains are recalculated) : Sequence: align-1nw1-query.scw PDB file : Tito_Scwrl_1NW1.pdb: