Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MQINPALAQAIEADSG--FELTQVEFIGAGWFAQAYRFCSQAKHYVVRISKHY---HDFLKDVYAYQHWGQ----QLPIPAILAHGQFADGWAYAISPHIAGQTIVSL-----EAAELRQIQPALFKSLQQLHQLKLGPVTGWGSVNSQGHGRYQSWAEEVLDIGNYKFDFDWHDWIKRDDQTGTLLRQGYRLMEQLLSTISTERSLIHRDFGFDNVLCQP-PTIVAVLDWADFGYGDWVYDIAQQSCYGQAEIYLQPWL--KFA-QQHDLI-PDNFEKRLHCYWLRMNLTDLIVSQLRNDWHWQRKIVAELSWLIENPVVVRLNQ 5IQC Chain:A ((10-285)) --TNVKAMKYLIEHYFDNFKVDSIEIIGSGYDSVAYLVN---NEYIFKTKFSTNKKKGYAKEKAIYNFLNTNLETNVKIPNIEYSYISDE-LSILGYKEIKGTFLTPEIYSTMSEEEQNLLKRDIASFLRQMHGLDYTDISECTID------NKQNVLEEYILLRET-----IYNDLTD--IEKDYIESFMERLNATT-VFEGKKCLCHNDFSCNHLLLDGNNRLTGIIDFGDSGIIDEYCDFIYLLEDS---EEEIGTNFGEDILRMYGNIDIEKAKEYQDIVEEYYPIETIVYGIKN---------------------------

General information: TITO was launched using: RESULT: Template: 5IQC.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1121 -7904 -7.05 -30.75 target 2D structure prediction score : 0.54 Monomeric hydrophicity matching model chain A : 0.66 3D Compatibility (PKB) : -7.05 2D Compatibility (Sec. Struct. Predict.) : 0.54 1D Compatibility (Hydrophobicity) : 0.66 QMean score : 0.326

(partial model without unconserved sides chains): PDB file : Tito_5IQC.pdb: (Unconserved sides chains are recalculated) : Sequence: align-5iqc-query.scw PDB file : Tito_Scwrl_5IQC.pdb: