Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MSTSQGEMRGRLRSNASSTGILSSNTYAKHTLKCSLKPEAMELIRCRHLNIQTTHVKAIELVSFNEAKNRLITKKVIGKELFHALWNPTNLLGRLQGHRLQNPDTLVHRITEIGTWLRKYHNSSAGLTPESTDGSW-LVTAFAQKIRG-IRASKLIPEPNLKKIEQKYSTELQKLT--TPDYLSFNNAFPCQVHGDFVLYNVLVDSQQNMHILDFSKTRISSNLEDVARFYSTLWAIAQTNRTRRSLLRELPTRFLKAYGLPPLIAETPYFRANLVYNFLAHLEAQHRGRKTFSWNTNREMGQITRAGMKWIYQQI 4FEW Chain:A ((97-254)) ----------------------------------------------------------------------LLTTAIPGKTAFQVL--------------EEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASDFDDERNGWPVEQVWK-EMHKLLPFSPDSVV--------THGDFSLDNLIFDEGKLIGCIDVGRVGIADRYQDLAILWNCLGEFSPS----------LQKRLFQKYGI-------------------------------------------------------

General information: TITO was launched using: RESULT: Template: 4FEW.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 534 -18021 -33.75 -117.02 target 2D structure prediction score : 0.66 Monomeric hydrophicity matching model chain A : 0.65 3D Compatibility (PKB) : -33.75 2D Compatibility (Sec. Struct. Predict.) : 0.66 1D Compatibility (Hydrophobicity) : 0.65 QMean score : 0.209

(partial model without unconserved sides chains): PDB file : Tito_4FEW.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4few-query.scw PDB file : Tito_Scwrl_4FEW.pdb: