Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MMKKVPEFVVQFSPESFSLTPVPGGLSNHNYR-LQFQQHGLNNRYFVRQFSATYLGMDNDVELEYAAQIQAANIGLA-PNVIVKHK--QGMICDWVTGEHWDQTAQ----VRDENIEKLAQLVAMLHQQPMPSHH-LDM---VER----LQHYYQTLNTEFKTVQLAQQLTLVIDLIEQHLPANRLGFCHHDMNPLNFIEDEQ----AKLYLLDWEFAAAGHCDFDIATLFQTFEW--QDAQQALFIRYYNQYYPAAKVTF-EQLDVMAVVVEMMTLLWCIVMYQQDKDATYLRLWQQSEYAITDKINRLSKELQWDQ 3CSV Chain:A ((7-306)) --DEIRDFLATHGYADWNRTP--------RYQRLRSPT---GAKAVLMDWSPEE---GGDTQPFVDLAQYLRNLDISAPEIYAEEHARGLLLIEDLGDALFTEVINNDPAQEMPLYRAAVDLLIHLHDAQTPELARLDPETLSEMTRLAFSEYRYAILGDAAEDNRKRFEHRFAQILSA-QLEGDMVFVHRDFHAQNLLWLPEREGLARVGVIDFQDAKLGHRAYDLVSLLQDARRDVPAQVEAQMIDHYIQATGVDESHFRSAYAVIAVQRNMRILGIFARLSQRFGKRHYIEFVPRVWAHFERGLAH---------

General information: TITO was launched using: RESULT: Template: 3CSV.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1230 -17734 -14.42 -65.92 target 2D structure prediction score : 0.65 Monomeric hydrophicity matching model chain A : 0.66 3D Compatibility (PKB) : -14.42 2D Compatibility (Sec. Struct. Predict.) : 0.65 1D Compatibility (Hydrophobicity) : 0.66 QMean score : 0.385

(partial model without unconserved sides chains): PDB file : Tito_3CSV.pdb: (Unconserved sides chains are recalculated) : Sequence: align-3csv-query.scw PDB file : Tito_Scwrl_3CSV.pdb: