Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MEEDRRHPRRQLDPALIASYLAG-RKIELVQLMPLGKSNTNYRLTLADGEVCLLRLHHPGA---NA-ERENHILRLVRE-IVPVPAVLASG-SDWSIHSFVEGI-PLADAPE-S-VHAAAEALARIASLRFTSSGWIQADGSIAPFDFGDGKSFVESMLERADVRAWVGADTAAALRRIEAAQPPAADDEPRLVHGDFNPTNILVHQRAVTGILDWEFSHAGSPYMDIGNLLRHTD--PAQHGALEAGLVAGGLNVPADWRQQAEMVDLSSHLEFLTTQRSDAFKQRCADWIRDFVERY 4R78 Chain:A ((20-253)) -------HMEKIIKEKISSLLSQEEEVLSVEQL-GGMTNQNYLAKTTN-KQYIVKFFGKGTEKLINRQDEKYNLELLKDLGLDVKNYLFDIEAGIKVNEYIESAITLDSTSIKTKFDKIAPILQTIHTSAKELRGEFAP-FEE----IKKYESLIEEQIPY----AN-YESVRNAVFSLEKRLA-DLGVDRKSCHIDLVPENFIESPQGRLYLIDWEYSSMNDPMWDLAALFLESEFTSQEEETFLSHYESDQT---------------------------------------------

General information: TITO was launched using: RESULT: Template: 4R78.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 918 7162 7.80 32.26 target 2D structure prediction score : 0.61 Monomeric hydrophicity matching model chain A : 0.67 3D Compatibility (PKB) : 7.80 2D Compatibility (Sec. Struct. Predict.) : 0.61 1D Compatibility (Hydrophobicity) : 0.67 QMean score : 0.302

(partial model without unconserved sides chains): PDB file : Tito_4R78.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4r78-query.scw PDB file : Tito_Scwrl_4R78.pdb: