Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MTGHGMAGDDWQYRKTMPQNILRPTVEAALPHV-----AGAEWEPLAGGRTNPVFKIN-AD----------APLVCKLSCLSGETPLFGNRALDEAAILKHLEGSGLAPSLTDVLAVPEGEILIYSFVEGDILTQ-------TIPAAVRILRQVHQAEIPDNLPRLDGS-SAAIIQKTKAILETLPV---------QKA---M-----AISFAEPKVELPDTLVPRLIHGDPVPANFIQSKGKLTLIDWQCPAIGDPCEDLAIALSPAMNAVYG----------DGPLTPQQMKECLNAYGD-RT----V--------SDRYRKLAPLFHWRMAAYCAWKVEQG---D-KDYAAGVKLELAALK 3C5I Chain:D ((11-349)) ---------------LTDPLYIKKICLEKVPEWNHFTEDNLRVKQIL--LTNQLFEVGLKEETANNYNSIRTRVLFRIYGK--HV-DELYNTISEFEVYKTMSKYKIAPQLLNTFN---G-GRIEEWLYGDPLRIDDLKNPTILIGIANVLGKFHTLSRKRHLP-EHWDRTPCIFKMMEKWKNQLFKYKNIEKYNCDIHKYIKESDKFIKFMKVYSKSDNLANTIVFCHNDLQENNIINTNKCLRLIDFEYSGFNFLATDIANFFIETSIDYSVSSYPFFEIDKKKYISYENRKLFITAYLSNYLDKVVPTPKLIDEILEAVEVQALGAHLLWGFWSIIRGYQTKSYNEFDFFLYAEQRL----

General information: TITO was launched using: RESULT: Template: 3C5I.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain D - contact count / total energy / energy per contact / energy per residue : 1021 -25095 -24.58 -93.99 target 2D structure prediction score : 0.67 Monomeric hydrophicity matching model chain D : 0.64 3D Compatibility (PKB) : -24.58 2D Compatibility (Sec. Struct. Predict.) : 0.67 1D Compatibility (Hydrophobicity) : 0.64 QMean score : 0.335

(partial model without unconserved sides chains): PDB file : Tito_3C5I.pdb: (Unconserved sides chains are recalculated) : Sequence: align-3c5i-query.scw PDB file : Tito_Scwrl_3C5I.pdb: