Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MRLLPNPERAWERAAHVQTLARAEIERRIPPFT----GEVEVLSGGHANVNVRLGA----ERVLRVYRRDA--SAAPKEASLLAHGWRS-FR-VPAVLSAGD---------DFLVLEHVPHVPLQDT------AMCGAAVGRALAEIHGVGYDSAGFLGPELAVSVPFADCIGALRGHVDAVLDR-LAPAL-RGELSERVRAFLDANAEALRALASAPVLLHGDFKASNLHWLS-SGELLVLDWEFAYAGPGLMDVGQLLRWSPGREFVAAFAGAYRESAPL--PEEFERWAAAFDLFNLVGLLDGVEPG-SRRAADVCGRISEVVER 5IGH Chain:A ((5-298)) -----------------TTADTSQLYALAARHGLKLHGPLTVNELGLDYRIVIATVDDGRRWVLRIPRRAEVSAKVEPEARVLAMLKNRLPFAVPDWRVANAELVAYPMLEDSTAMVIQPGSSTPDWVVPQDSEVFAESFATALAALHAVPISAAVDAGM-LI--RT----PTQARQKVADDVDRVRREFVVNDKRLHRWQRWLDDDSS----WPDFSVVVHGDLYVGHVLIDNTERVSGMIDWSEARVDDPAIDMAAHLMVFG-EEGLAKLLLTYEAAGGRVWPRLAHHIAERLAFGAVTYALFALDSGNEEYLAAAKAQLA-----

General information: TITO was launched using: RESULT: Template: 5IGH.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1095 -6433 -5.87 -24.65 target 2D structure prediction score : 0.68 Monomeric hydrophicity matching model chain A : 0.65 3D Compatibility (PKB) : -5.87 2D Compatibility (Sec. Struct. Predict.) : 0.68 1D Compatibility (Hydrophobicity) : 0.65 QMean score : 0.311

(partial model without unconserved sides chains): PDB file : Tito_5IGH.pdb: (Unconserved sides chains are recalculated) : Sequence: align-5igh-query.scw PDB file : Tito_Scwrl_5IGH.pdb: