Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MNQAQFEKVIEVLFPDIIIQEVTAYEKGWDNDIFIVN----KEIVFRFPRSEEVASKVKDEIILLKHLAHKEPKLDIPRYKGIEMD-GNLKC----VKYNFMKGKSLSEIKNSRLDLHSAELLGDFLSRLHSIELSALKETNITSFHTDTYWENLYESAKTYVFPNITHSERAEIQQFYEDFSNNPIYSNVNKAVIHGDLTAANILYNKEEECVSGIIDFTDAQIADPAFDFAGLYWAYGPEFTKKLLTYYDGKD---KTGIFERVQSFYGLQPIFHELIYAVKENHDVDWGSALERFQYLNSHR 5IGI Chain:A ((8-298)) -DTSQLYALAAR-HGLKLHGPLTVNELGLDYRIVIATVDDGRRWVLRIPRRAEVSAKVEPEARVLAMLKNR-LPFAVPDWRVANAELVAYPMLEDSTA-VIQPGSSTPDWVVPQDSEVFAESFATALAALHAVPISAAVDAGMLIRTP-TQARQKVADDVDRVRREFVV-NDKRL-HRWQRWLDDDSSWPDFSVVVHGDLYVGHVLIDN-TERVSGMIDWSEARVDDPAIDMAAHLMVFGEEGLAKLLLTYEAAGGRVWPRLAHHI-AERLAFGAVTYALFALDSGNEEYLAAAKAQLA------

General information: TITO was launched using: RESULT: Template: 5IGI.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1271 3311 2.61 11.91 target 2D structure prediction score : 0.68 Monomeric hydrophicity matching model chain A : 0.70 3D Compatibility (PKB) : 2.61 2D Compatibility (Sec. Struct. Predict.) : 0.68 1D Compatibility (Hydrophobicity) : 0.70 QMean score : 0.479

(partial model without unconserved sides chains): PDB file : Tito_5IGI.pdb: (Unconserved sides chains are recalculated) : Sequence: align-5igi-query.scw PDB file : Tito_Scwrl_5IGI.pdb: