Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MRLLPNPERAWERAAHVQTLARAEIERRIPPFT----GEVEVLSGGHANVNVRLGA----ERVLRVYRRDA--SAAPKEASLLAHGWRS-FR-VPAVLSAGD---------DFLVLEHVPHVPLQDT------AMCGAAVGRALAEIHGVGYDSAGFLGPELAVSVPFADCIGALRGHVDAVLDR-LAPAL-RGELSERVRAFLDANAEALRALASAPVLLHGDFKASNLHWLS-SGELLVLDWEFAYAGPGLMDVGQLLRWSPGREFVAAFAGAYRESAPL--PEEFERWAAAFDLFNLVGLLDGVEPG-SRRAADVCGRISEVVER 5IGI Chain:A ((5-298)) -----------------TTADTSQLYALAARHGLKLHGPLTVNELGLDYRIVIATVDDGRRWVLRIPRRAEVSAKVEPEARVLAMLKNRLPFAVPDWRVANAELVAYPMLEDSTA-VIQPGSSTPDWVVPQDSEVFAESFATALAALHAVPISAAVDAGM-LI--RT----PTQARQKVADDVDRVRREFVVNDKRLHRWQRWLDDDSS----WPDFSVVVHGDLYVGHVLIDNTERVSGMIDWSEARVDDPAIDMAAHLMVFG-EEGLAKLLLTYEAAGGRVWPRLAHHIAERLAFGAVTYALFALDSGNEEYLAAAKAQLA-----

General information: TITO was launched using: RESULT: Template: 5IGI.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1152 -11669 -10.13 -44.88 target 2D structure prediction score : 0.66 Monomeric hydrophicity matching model chain A : 0.64 3D Compatibility (PKB) : -10.13 2D Compatibility (Sec. Struct. Predict.) : 0.66 1D Compatibility (Hydrophobicity) : 0.64 QMean score : 0.318

(partial model without unconserved sides chains): PDB file : Tito_5IGI.pdb: (Unconserved sides chains are recalculated) : Sequence: align-5igi-query.scw PDB file : Tito_Scwrl_5IGI.pdb: