Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MNLEKWIEEKFNTTKYTLEKTDKGISNHNYILTIN--DTKYMIRVPKKNHESLGLQHEHEKKILTL--VSDLDVPTVLFDEKEGIKVTHYIEDVKTFNECK-DKDKFARCANLMKSLHTKKAPLF---I------------FNPFGKIEFYKSQIKECIV---------------SFPNE---ENFLEALKKE-YKPNTLCHNDFVQGNILYSD--TKDYLIDYEYAAKNDYRFDIASFFSENNI---------HYIDQRDQFYQTYFDG-EID--PMIDVQVQAFERMEDILWGYWANMLYEQRGEQIYFDIAKDKEKHYRG 2QG7 Chain:E ((110-449)) --------------SLEFQIING-ITNILIKVKDMSKQAKYLIRLYGPKTDEI-INREREKKISCILYNKNIAKKIYVFFTN--GRIEEFMDGYALSREDIKNPKFQKLIAKNLKLLHDIKLNENLYKELQVTQKVPGTRPSFLWNTIWKYFHLLNEERKKICSFDAKANILKLIDFDVLRDSIVEVESLCKRENSPIVLCHCDLLSSNIINTVGGDSISFIDFEYSCPMERAYDIANHFNEYAGFNCDWDLTPSKEEE-YHFIMHYLGTDDEELINQLIREIQPFYICSHINWGLWSLLQGMHSSDFDFINYGMTRLTAS--

General information: TITO was launched using: RESULT: Template: 2QG7.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain E - contact count / total energy / energy per contact / energy per residue : 980 37743 38.51 151.58 target 2D structure prediction score : 0.65 Monomeric hydrophicity matching model chain E : 0.62 3D Compatibility (PKB) : 38.51 2D Compatibility (Sec. Struct. Predict.) : 0.65 1D Compatibility (Hydrophobicity) : 0.62 QMean score : 0.255

(partial model without unconserved sides chains): PDB file : Tito_2QG7.pdb: (Unconserved sides chains are recalculated) : Sequence: align-2qg7-query.scw PDB file : Tito_Scwrl_2QG7.pdb: