Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MHSRPQISPERAAQLAAEWFEGQLDAPAELDSYADRNFLVRAPDGTKAVLKVPNVELAEDIDLQIAILKWLEARPSAPLVPRVLGPTRTIEDDAGRPTRAWMVGWIEGELWFDASPTPALREELGAALGQLARDLEDFRHPGMERHFAWNLAEANWIAEELHRFEDPARAELVCDALMQFQGRVLPRLAELPTQVIHGDANDKNLAVAEGRLVGVFDFGDACWCPAVCDLAIALAYAAMDLRLGCALPEVAAACAQVCAGYHGRRPLTALELDLLYDLLRARLAVSVTSSTRAREAEPDNAYVSASEQGAWSVLEALGAVGRRAFTATVAEACGLALAPRVPGARTGDELLAARRRRLGPSLSLSYASSGMPLYIRRGEGSWLFDEHDQAFLDCVN--NVCHVGHCHPRVVEA---------GAAQMARLNTNTRYLHEGLVDYAEALCATLPA-PLEVVYLVNSGSEANELALRLARDYTGGFDVAVLDAAYHGNTGNLVDMSPYKFDAPGGRGRREWVHVLPTPDPYRGAHGDDGPAYAAALDAADAAARGRGSRLAALFCESVLGCAGQVVPASGFLAAAYARARAAGAVCIADEVQVGFGRVGDGMWAFEAEGVVPDILTLGKPIGNGHPLGAVVTTRAIAEALGGGRMEFFCTFGGNPVSAAVGAAVLAVIEDEGLVANARDTGSWLRGAFEQLAADPVLGRGIGEVRGRGLFIGVELVEDRSTKRPDAARASAIVAHARARGVLLSTDGPARNVIKIKPPICFADVEARILASTLARALSATMR 5M49 Chain:A ((22-421)) ----------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------FPLAVAGGQGARLVEEDGRELIDLSGAWGAASLGYGHPAIIEAVSRAAANPAGASILSASNAPA-------VALAERLTASFP-RGTHKVWFGHSGSDANEAAYRAITRATGRTGVIAFIGAYHGCTVGSMAFS---------KA--DGLILLPYPDPYRPYQDDPTGDAVLALLKERLAAVPAGS-IAAAFIEPIQSDGGLIVPPDGFLRKFADICRAHGISVVCDEVKVGLARSGR-LHCFEHEGFVPDILVLGKGLGGGLPLSAVIAPAEILDCASAFAMQ---TLHGNPVCAAAGLAVLETIEAENLTTAAERKGKLLREGLARLAERHEL---IGDIRGRGLACGVELVRNRQSREPARAETAKLIYRAYELGLVLYYVGMNGNVLEMTPPLTMTEDEVRHAVNLLDQAFT----

General information: TITO was launched using: RESULT: Template: 5M49.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 2261 -24296 -10.75 -64.45 target 2D structure prediction score : 0.63 Monomeric hydrophicity matching model chain A : 0.65 3D Compatibility (PKB) : -10.75 2D Compatibility (Sec. Struct. Predict.) : 0.63 1D Compatibility (Hydrophobicity) : 0.65 QMean score : 0.241

(partial model without unconserved sides chains): PDB file : Tito_5M49.pdb: (Unconserved sides chains are recalculated) : Sequence: align-5m49-query.scw PDB file : Tito_Scwrl_5M49.pdb: