Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MEEDRRHPRRQLDPALIASYLAGR------KIELVQLMPLGKSNTNYRLTLAD--------GEVCLLRLHHPGA--NA-ERENHILRLVRE-IVPVPAVLASGSDWSIHSFVEGIPLADAP---ES-VHAAAEALARIASLRFTSSGWIQADGSIAPFDFGDGKSFVESMLERAD-V---RAWVGADTAAALRRIEAA---QPPAADDEPRLVHGDFNPTNILVHQR------AVTGILDWEFSHAGSPYMDIGNLLRHTD--------------------PAQHGALEAGLVAGGLNVPADWRQQAEMVDLSSHLEFLTTQRSDAFKQRCADWIRDFVERY 5FUT Chain:A ((6-308)) ------PRTRRRAYLWCKEFLPGAWRGLREDEFHISVIRGGLSNMLFQCSLPDTTATLGDEPRKVLLRLYG---GAEAMVLESVMFAILAERSLG-PKLYGIFPQGRLEQFIPSRRLDTEELSLPDISAEIAEKMATFHGMKMPFNKEPKWL-------FGTMEKYLKEVLRIKFTEESRIKKLHKLLSYNLPLELENLRSLLESTPSPVVFCHNDCQEGNILLLEGRENSEKQKLMLIDFEYSSYNYRGFDIGNHFCEWMYDYSYEKYPFFRANIRKYPTKKQQLHFISSYLPAFQ---------------------------------------------

General information: TITO was launched using: RESULT: Template: 5FUT.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 730 -12048 -16.50 -53.55 target 2D structure prediction score : 0.64 Monomeric hydrophicity matching model chain A : 0.63 3D Compatibility (PKB) : -16.50 2D Compatibility (Sec. Struct. Predict.) : 0.64 1D Compatibility (Hydrophobicity) : 0.63 QMean score : 0.278

(partial model without unconserved sides chains): PDB file : Tito_5FUT.pdb: (Unconserved sides chains are recalculated) : Sequence: align-5fut-query.scw PDB file : Tito_Scwrl_5FUT.pdb: