Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MCINFLVCASLSMFRTSVSGARVPIGAWPDRGGEVRDAVLSVFRRNEYMREIDTGARVASPGTGCDGLVGTCPVDKPVDSVAVGVLARFLAQLTLVRKEALPALPAEWSRNERDSRAYLDTLVRHAKRRMLDPNWTAIGGLFAVLGVPGDRLVRFAEQVPAMSRRPYGLLLTNLARDNVTILEGPKLAEAGLDWTSASYGDPLHGLAGHVVRMRYPQDQVNEVVQAWACEMRRIRPPAVSGLDRDLPYYLDFERIHAAYDDVIRAARSLGDSIENLALLAAASDIGTALKRAAGLLGMSEVPPEKEIADALFRWQAARLVRAAERVPDSLFRWRCDDRVPERDDFPSDLVRRALAVEG--VAPADRVFRGTAHLNTVVQLENYPDPVVVRRETAAAPRREQGLLPEHAVLQVIERS---GVQVRAPRILALGYDDRRRHVAIHTYEGPGGRSPQHPVDGLLPAEADELVDELAALAEVDHSSLPSNEPEGGFYRWLAERLADFVAALPDATLRAADQRGLPDGDLLRQILGRYKVSDRAPVLLHGDLNPWNLVRAGRQGGLTIIDWEMGMIGDPLYDLVRHLH-LTPTRTEMRVRMFDRWSKKLPAECVVGWEEDWRVYRYIEQIRSAYVDLDRMTTGAALS-TPNVRRAVDAYEMTLSAATGSLGLRRRVVPKVPASLKVS---- 5UFL Chain:A ((5-561)) -------TACLKSLLLTVSQYKA--------------------NATQLLRHLEVISRLFTS-------------NQILTSECLSCLVELLEDPSASLILSIIGLLSQLAVDIETRDCLQNTL---------------NSVLAGVVCRTDSVFLQCIQLLQKLT---YN---VKIFYSGANI---DELITFLIDHIQSELKMPCLGLLANLCRH-------NLSVQTHIKTLS-------NSFYRTLITLLAHSSLTVVVF-ALSILSSLTLNEEVGEKLFHARNIHQTFQLIFNILINGDGTLTRKYSVDLLMDLLKN-----PKIADYLTRY---------EHFSSCLHQVLGLLNGKDPDSSSKVLELLLAFCSVTQLRHMLTQMMFESHT-------KCLEPTVALLRWLSQPLDGNCSVLALELFKEIFEDVI--------DAANCSSADRFVTLLLPTILDQL-------------QFTEQNLDEALTRKKCERIAKAIEVLLTLC----------GDDTLKMHIAKILTTVKCTTLIEQQFTYGKIDL--GFGTKVADSELCKLAADVILKTLDLINKLKPLVPGMEVSFYKILQD------------------------------PRLITPLAFALTSDNREQVQSGLRILLEAAPLPDFPALVLGESIAANNAYRQQE

General information: TITO was launched using: RESULT: Template: 5UFL.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 2236 -171590 -76.74 -335.14 target 2D structure prediction score : 0.57 Monomeric hydrophicity matching model chain A : 0.66 3D Compatibility (PKB) : -76.74 2D Compatibility (Sec. Struct. Predict.) : 0.57 1D Compatibility (Hydrophobicity) : 0.66 QMean score : 0.191

(partial model without unconserved sides chains): PDB file : Tito_5UFL.pdb: (Unconserved sides chains are recalculated) : Sequence: align-5ufl-query.scw PDB file : Tito_Scwrl_5UFL.pdb: