Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MNLEKWIEEKFNTTKYTLEKTDKGISNHNYILTIN--DTKYMIRVPKKNHESLGLQHEHEKKILTL--VSDLDVPTVLFDEKEGIKVTHYIEDVKTFNECK-DKDKFARCANLMKSLHTKKAPLF---I------------FNPFGKIEFYKSQIKECIV---------------SFPNE---ENFLEALKKE-YKPNTLCHNDFVQGNILYSD-TKDYLIDYEYAAKNDYRFDIASFFSENNI---------HYIDQRDQFYQTYFDG-EID--PMIDVQVQAFERMEDILWGYWANMLYEQRGEQIYFDIAKDKEKHYRG 2QG7 Chain:D ((110-449)) --------------SLEFQIINGG-TNILIKVKDMSKQAKYLIRLYGPKTD----NREREKKISCILYNKNIAKKIYVFFTN--GRIEEFMDGYALSREDIKNPKFQKLIAKNLKLLHDIKLNENLYKELQVTQKVPGTRPSFLWNTIWKYFHLLNEERKKICSFDAKANILKLIDFDVLRDSIVEVESLCKRENSPIVLCHCDLLSSNIINTVGDSISFIDFEYSCPMERAYDIANHFNEYAGFNCDWDLTPSKEEE-YHFIMHYLGTDDEELINQLIREIQPFYICSHINWGLWSLLQGMHSSDFDFINYGMTRLTAS--

General information: TITO was launched using: RESULT: Template: 2QG7.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain D - contact count / total energy / energy per contact / energy per residue : 960 44718 46.58 181.78 target 2D structure prediction score : 0.64 Monomeric hydrophicity matching model chain D : 0.61 3D Compatibility (PKB) : 46.58 2D Compatibility (Sec. Struct. Predict.) : 0.64 1D Compatibility (Hydrophobicity) : 0.61 QMean score : 0.261

(partial model without unconserved sides chains): PDB file : Tito_2QG7.pdb: (Unconserved sides chains are recalculated) : Sequence: align-2qg7-query.scw PDB file : Tito_Scwrl_2QG7.pdb: