Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MLACGSDDLRTEEGLRIAALYGWDPED-TACQLLQRGENGTFLVR-TSGTRYILRRYRRERYREQEILAELAWMCALR-DHINVPNVIANTEGDLATRLVGPDGERLYAVFEFIEGESPESSSSEDYRRLGELMKALHRGADAIAQSMNEDWAGWNRPVYDVQRAVREPLRHLLQFEALSEVNKTRCVSVARELERRFRT---LQPGRPFVHADLHFGNIVVREPNWYCLDFDECGYGHRAFDLGVVRLHLRNRSDNFDCWAHFEHGYGDMPSRGDLATGTAARIFYMAGKIPMRQDIDSLRHNPDARIGKYLTWIEDELM 1ZYL Chain:A ((8-285)) -----FQTLHPDTIMDALFEHGIR--VDSGLTPLNSYENRVYQFQDEDRRRFVVKFYRPERWTADQILEEHQFALQLVNDEVPVAAPVA-FNGQTLLNH----QGFYFAVFPSVGGRQFEADNIDQMEAVGRYLGRMHQTGRKQL--FIH------RPTIGLNEYLIEPRKLFEDATLIPSGLKAAFLKATDELIAAVTAHWREDFTVLRLHGDCHAGNILWRD-GPMFVDLDDARNGPAVQDLWMLLNGDKA--EQRMQLETIIEAYEEFSEFDTAEI-GLIEPLRAMRLVYYLAWLMRRW-------------------

General information: TITO was launched using: RESULT: Template: 1ZYL.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1234 -23236 -18.83 -85.43 target 2D structure prediction score : 0.49 Monomeric hydrophicity matching model chain A : 0.67 3D Compatibility (PKB) : -18.83 2D Compatibility (Sec. Struct. Predict.) : 0.49 1D Compatibility (Hydrophobicity) : 0.67 QMean score : 0.261

(partial model without unconserved sides chains): PDB file : Tito_1ZYL.pdb: (Unconserved sides chains are recalculated) : Sequence: align-1zyl-query.scw PDB file : Tito_Scwrl_1ZYL.pdb: