Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MAKPELIELATSCFGL--TVDEQLPIGESFASEVLRFRS-GRQCYVLKRPFSL---EKAEREYFWLKRLSH-C--PFVPDALEITKANDHGLILMTSLEGMPLKSFHQ-------------LSHRDLSKLGHDLRALHTVSADSFDGHK----SWRELLLSNTDRYIDKIIGPARKTAEMAYARFHEGIDE-IPDSYLPTATHFDFRDGNILADES-GYTGIIDFESMRGGHASMDFFKLLTHPSDMNDIELTALLAGYGSADWLESM--AQLKHLVACYAVYHGLAGLAWCSQREQTSTEFYQRCAGFLEQGR 5IWU Chain:A ((4-299)) -DIKQVIEIAKK-HNLFLKEETIQFNESGLDFQAVFAQDNNGIDWVLRLPRREDVMPRTKVEKQALDLVNKYAISFQAPNWIIYT----EELIAYKKLDGVPAGTIDHNIGNYIWEIDINNVPELFHKSLGRVLAELHSIPSNKAAALDLVVHTPEEA-RMSMKQRMDAVRAKFGV-GENLWNRWQAWLNDDDMWPKKTGLIHGDVHAGHTMIDKDANVTGLIDWTEAKVTDVSHDFIFNYRAF---GEEGLEALILAYKEI---GGYYWPKMKEHIIELNAAYPVSIAEFALVSG--IEEYEQMAKEALEV--

General information: TITO was launched using: RESULT: Template: 5IWU.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1196 24618 20.58 92.55 target 2D structure prediction score : 0.67 Monomeric hydrophicity matching model chain A : 0.64 3D Compatibility (PKB) : 20.58 2D Compatibility (Sec. Struct. Predict.) : 0.67 1D Compatibility (Hydrophobicity) : 0.64 QMean score : 0.386

(partial model without unconserved sides chains): PDB file : Tito_5IWU.pdb: (Unconserved sides chains are recalculated) : Sequence: align-5iwu-query.scw PDB file : Tito_Scwrl_5IWU.pdb: