Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MSQVDTLDTQKLAEYLEQHIDGFKGPIEADKFEGGQSNPTFKITAASGAYVLRRQPPGKLLKSAHAVDREFRVMAALKDT---DVPVPNVLHLCEDRDVIGSMFYVMEFCEGRILWSAGLPEMGEGEAANAVRGEMYSEMNRVLAAMHSVDLEAVGLTDYGKPGNYFERQLGRWTQQYEA-SKLQEIPAMDSLIEWLQANQPEDDGQVSLVHGDYRLDNMMWHPEKPQVIAVLDWELSTLGHPLADLAYQCMGMRMPQRGAKMAGLQGKDRTALGIPTEKEYVAMYCERRGISQIDNWEFYLAFSFFRLAAICQGVAKRAEDGNASNKQAAEVGALVEPLATMAMQIINEGA 5IQC Chain:A ((8-257)) ----NATNVKAMKYLIEHYFDNF-KVDSIEIIGSGYDSVAYLV---NNEYIFKTKFSTN---KKKGYAKEKAIYNFLNTNLETNVKIPNIEYSYIS---DELSILGYKEIKGTFLTPEIYSTMSEE-----EQNLLKRDIASFLRQMHGLDYTDISECTIDNKQNVLEE-YILLRETIYNDLTDIEKDYIESFMERLNATTV-FEGKKCLCHNDFSCNHLLLDGN-NRLTGIIDFGDSGIIDEYCDFIYLLEDSEEE----------------IGTNFGEDILRMYGN----------------------------------------------------------------

General information: TITO was launched using: RESULT: Template: 5IQC.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1019 -15796 -15.50 -64.21 target 2D structure prediction score : 0.54 Monomeric hydrophicity matching model chain A : 0.67 3D Compatibility (PKB) : -15.50 2D Compatibility (Sec. Struct. Predict.) : 0.54 1D Compatibility (Hydrophobicity) : 0.67 QMean score : 0.283

(partial model without unconserved sides chains): PDB file : Tito_5IQC.pdb: (Unconserved sides chains are recalculated) : Sequence: align-5iqc-query.scw PDB file : Tito_Scwrl_5IQC.pdb: