Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MTRDALDRAAVTAFLVARHGDAVCDSAIEIRPLPGGLCSEVALVTARLRGAAGARRVTLVAKLV-H-----GTAAREAAVYRRLSRSVGRRFSPELLGAERLGRGRWLLYLERIEPAH-AWPWAD-SGHTARVLERLARLHEETPQGAALPAWDYEAENLAMARDTLSLAERLPRGEVPALARALPALRRVVSALPAMRRQLLAFQPLGRAVIHGDVHPGNVMVRRRDGVVEPVLLDWARARMGSPLEDVSSWLQALGYWEPEARRRHDTLLAGYLSARGLPPALTRPLRDVYWFASASNALAGALGHHLAMACGASAPGVLAAAARNAADWLRVIRRADACWS 4R78 Chain:A ((18-253)) ---GSHMEKIIKEKISSLLSQE--EEVLSVEQL-GGMTNQNYLAKTTN--------KQYIVKFFGKGTEKLINRQDEKYNLELLKDL--GLDVKNYLFDI----EAGIKVNEYIE-SAITLDSTSIKTKFDKIAPILQTIHTSAKELRG--E----FAPFEEIKKYESLIEEQ----IPYA-----NYESVRNAVFSLEKR-LADLGVDRKSCHIDLVPENFIESP-Q--GRLYLIDWEYSSMNDPMWDLAALFLES-EFTSQEEET---FLSHYESDQT----------------------------------------------------------------

General information: TITO was launched using: RESULT: Template: 4R78.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 955 6391 6.69 28.03 target 2D structure prediction score : 0.62 Monomeric hydrophicity matching model chain A : 0.62 3D Compatibility (PKB) : 6.69 2D Compatibility (Sec. Struct. Predict.) : 0.62 1D Compatibility (Hydrophobicity) : 0.62 QMean score : 0.209

(partial model without unconserved sides chains): PDB file : Tito_4R78.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4r78-query.scw PDB file : Tito_Scwrl_4R78.pdb: