Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MCINFLVCASLSMFRTSVSGARVPIGAWPDRGGEVRDAVLSVFRRNEYMREIDTGARVASPGTGCDGLVGTCPVDKPVDSVAVGVLARFLAQLTLVRKEALPALPAEWSRNERDSRAYLDTLVRHAKRRMLDPNWTAIGGLFAVLGVPGDRLVRFAEQVPAMSRRPYGLLLTNLARDNVTILEGPKLAEAGLDWTSASYGDPLHGLAGHVVRMRYPQDQVNEVVQAWACEMRRIRPPAVSGLDRDLPYYLDFERIHAAYDDVIRAARSLGDSIENLALLAAASDIGTALKRAAGLLGMSEVPPEKEIADALFRWQAARLVRAAERVPDSLFRWRCDDRVPERDDFPSDLVRRALAVEGVAPADRVFRGTAHLNTVVQLENYPDPVVVRRETAAAPRREQGLLPEHAVLQ--VIERSGVQVRAPRILALGYDDRRRHVAIHTYEGPGGRSPQHPVDGLLPAEADELVDELAALAEVDHSSLPSNEPEGGFYRWLAERLADFVAALPDATLRAADQRGLPDGDLLRQILGRYKVSDRAPVLLHGDLNPWNLVRAGRQGGLTIIDWEMGMIGDPLYDLVRHLHLTPTRTEMRVRMFDRWSKKLPAECVVGWEEDWRVYRYIEQIRSAYVDLDRMTTGAALSTPNVRRAVDAYEMTLSAATGSLGLRRRVVPKVPASLKVS 5E75 Chain:A ((13-519)) --------FYTSDEALEAATAPLYNRAWFDYNQRSIVPIGS--GRANDMYSPWNYPQFVTFQVT------------ALDENLSGAWSGFYSVVTMANSVINAVETQTQSV----------SEAAKTKAIAEARLMRACAYFYML--------------------RIWGPVI--LIEDNQKLVD-----------------NPVRPLNREEDVFQFIINDLNYAVDNLSEQSDK-------------------GRATSWAAKGILAKVYLARSGWNNGGTRDEGDLELARQYASDVCENSGLDLMTNYEDLFKYKNNNN------QESLLAMQWVPLGEWYECNTLLSDLAFSTEVTGGVNCWSS-YNGSIDMLQQYELAD-------------TLRRNATFFTKGSYYSYICIKDGGYTYKGTA-------------SPIKKGVPGG-----PDDDN------------------DGKVKQMNSPLNTYILRLADVYLTYAEACLGNNSTLSDGRGL---YFFNRVRERAKINKKSSI--------------TLDDIIRERRVEFGMEYSNWYDMVTWFRYLPDKMLNYFNNQWRGYRT---DAIIKDEDGKLHFGKYDTDGTTFLEGPEYYTAPEF-TINIEAEDIFLPYPESDVIQNPLLNEPPVPYTFNE----

General information: TITO was launched using: RESULT: Template: 5E75.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 2880 -54633 -18.97 -108.40 target 2D structure prediction score : 0.50 Monomeric hydrophicity matching model chain A : 0.62 3D Compatibility (PKB) : -18.97 2D Compatibility (Sec. Struct. Predict.) : 0.50 1D Compatibility (Hydrophobicity) : 0.62 QMean score : 0.143

(partial model without unconserved sides chains): PDB file : Tito_5E75.pdb: (Unconserved sides chains are recalculated) : Sequence: align-5e75-query.scw PDB file : Tito_Scwrl_5E75.pdb: