Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MAKPELIELATSCFGLTVDEQLPIGESFASEVLRFRS-GRQCYVLKRPFSL-----EKAEREYFWLKRLSH--CPFVPDALEITKANDHGLILMTSLEGMPLKSFHQLSHRDLSKLGHDLRALHTVSADSFDG---------------HKSWRELLLSN-TDRYIDKIIGPA--RKTAEMAYARFHEGIDEI-PDSYLPTATHFDFRDGNILADESGYTGIIDFESMRGGHASMDFFKLLTHPSDMNDIELTALLAGYGSADWLESM-AQLKHLVACYAVYHGLAGLAWCSQREQTSTEFYQRCAGFLEQGR 3F7W Chain:A ((2-284)) --VNSVAARVTELTGREVAAVAERGHSHRWHLYRVELADGTPLFVKALPDDAPALDGLFRAEALGLDWLGRSFGSPVPQVAGWD----DRTLAMEWVDERPPT------PEAAERFGHQLAAMHLAGAESFGATWDGYIGPLPMDNTPRSTWPEFYAEQRILPYLRRAADRGALTPGDVRLVEKVLDALDHLAGDPEPPARIHGDLWNGNVLWQDDG-AVVIDPA-AHGGHREADLAMLALF----GLPYLDRVRDAYNEV---APLAEGWRARIPLHQLHPLLVHVCLF------GAAYRTTLVDTARA--

General information: TITO was launched using: RESULT: Template: 3F7W.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1288 -37062 -28.77 -145.34 target 2D structure prediction score : 0.67 Monomeric hydrophicity matching model chain A : 0.66 3D Compatibility (PKB) : -28.77 2D Compatibility (Sec. Struct. Predict.) : 0.67 1D Compatibility (Hydrophobicity) : 0.66 QMean score : 0.469

(partial model without unconserved sides chains): PDB file : Tito_3F7W.pdb: (Unconserved sides chains are recalculated) : Sequence: align-3f7w-query.scw PDB file : Tito_Scwrl_3F7W.pdb: