Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MTAAEAGADTDAALLALAQRLAAQAGKATPTALTRLAGGRNNQVFR-VDTEA-GPLVLKRYFTDARDSRDRLGAEWSFISHAWSRGVRVVPEPMACDRAVQAGLYSFVEGRKLTAAEL-----APAHVDAAIDFVLAVNAPPRPTLAPGSEACFSLAEHIATVERRVARLATLDADVPHVSEARQFVATRLQPAWAEVKATILRGAAAEGLALDAAIPADQVCLSPSDFGFHNALIDDQ----GKLTFLDFEYAGRDDPAKLVSDFFCQPEVPVPLAAHEHFIDRLADGLGLDAASVARCRLLLDAYQVKWTCIILNDFLPLGAVRRAFADTGAWAQRCAVQLAKAEAQLGLIGATSD 3CSV Chain:A ((8-259)) ----------------EIRDFLAT-HGYADWNRTP--------RYQRLRSPTGAKAVLMDWSPEE---GGDTQPFVDLAQYLRNLDIS-APEIYAEEHARGLLLIEDLGDALFTEVINNDPAQEMPLYRAAVDLLIHLHDAQTPELARLD-----PETLSEMTRLAFSEYRYAILG------------DAAEDNRKRFEHRFAQILS--------AQLEGDMVFVHRDFHAQNLLWLPEREGLARVGVIDFQDAKLGHRAYDLVSLLQDARRDVPAQVEAQMIDHYIQATGVDESHFR------------------------------------------------------------

General information: TITO was launched using: RESULT: Template: 3CSV.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 980 -5754 -5.87 -24.70 target 2D structure prediction score : 0.61 Monomeric hydrophicity matching model chain A : 0.63 3D Compatibility (PKB) : -5.87 2D Compatibility (Sec. Struct. Predict.) : 0.61 1D Compatibility (Hydrophobicity) : 0.63 QMean score : 0.243

(partial model without unconserved sides chains): PDB file : Tito_3CSV.pdb: (Unconserved sides chains are recalculated) : Sequence: align-3csv-query.scw PDB file : Tito_Scwrl_3CSV.pdb: