Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MRSEALEQFVVEEAGVGAPRGASWSRVDEGQGANRVWRVRWGPGSSVIVKRCGPGRAFVQERRAATRWAPAVADASRGLGHARVLAASQA---LRC----LVFVDA-GRGEEQV---ALSPAAFACAGRYLARLHALPVDERDPVP----LVEALARREARWLEGAPE----LRAVASSLGPGALAD-A-DWGPRVPCHRDFQPDNWLWDG--RTLTVVDFEHARPDARALDLAKLWTRLDGDVDARANAAFWSAYLGPEPCS-PSFLAQLRAGLVLHGLASVAWGRSHDDEAFVAEGERAVALARSGREPPNPRAGTPLASL 5IGH Chain:A ((11-300)) ----QLYALAAR-HGLKL--HGPLTVNE-LGLDYRIVIATVDDGRRWVLRIPRRAEV-SAKVEPEARVLAMLKNR-LPFAVPDWRVANAELVAYPMLEDSTAMVIQPGSSTPDWVVPQDSEVFAESFATALAALHAVPISAAVDAGMLIRTPTQARQKVADDVDRVRREFVVNDKRLHRW-QRWLDDDSSWPDFSVVVHGDLYVGHVLIDNTERVSGMIDWSEARVDDPAIDMAAHLMVFG----EEGLAKLLLTYEAAGGRVWPRLAHHIAERLAFGAVTYALFALDSGNEEYLAAAKAQLAAA------------------

General information: TITO was launched using: RESULT: Template: 5IGH.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1189 -41019 -34.50 -154.21 target 2D structure prediction score : 0.58 Monomeric hydrophicity matching model chain A : 0.66 3D Compatibility (PKB) : -34.50 2D Compatibility (Sec. Struct. Predict.) : 0.58 1D Compatibility (Hydrophobicity) : 0.66 QMean score : 0.294

(partial model without unconserved sides chains): PDB file : Tito_5IGH.pdb: (Unconserved sides chains are recalculated) : Sequence: align-5igh-query.scw PDB file : Tito_Scwrl_5IGH.pdb: