Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MRLLPNPERAWERAAHVQTLARAEIERRIPPFT----GEVEVLSGGHANVNVRLGAE----RVLRVYRRDA----SAAPKEASLLAHGWRS-FR-VPAVLSAGDDFLVLEHVPHVPLQDTAMCGAAVGRALAEIHGVGYDSAGFLGPE--LAVSVPFADCIGALRGHVDAV-LDR-LAPALR----GELSERVRAFLDANAEALRALASAPVLLHGDFKASNLHWLSSGELLVLDWEFAYAGPGLMDVGQLLRWSPGREFVAAFAGAYRESAPLPEEFERWAAAFDLFNLVGLLDGVEPGSRRAADVCGRISEVVER 3F7W Chain:A ((2-286)) ---------------------VNSVAARVTELTGREVAAVAERGHSHRWHLYRVELADGTPLFVKALPDDAPALDGLFRAEALGLDWLGRSFGSPVPQVAGWDDRTLAMEWVDERPPTP--EAAERFGHQLAAMHLAGAESFG-ATWDGYIGPLPMDNTPRSTWPEFYAEQRILPYLRRAADRGALTPGDVRLVEKVLDALDHLAGDPEPPARIHGDLWNGNVLWQDD-GAVVIDPA-AHGGHREADLAMLALFG--LPYLDRVRDAYNEVAPLAEGWRARIPLHQLHPLLVHVCLFG--AAYRTTLVDTARAAL--

General information: TITO was launched using: RESULT: Template: 3F7W.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1386 -8885 -6.41 -33.78 target 2D structure prediction score : 0.63 Monomeric hydrophicity matching model chain A : 0.64 3D Compatibility (PKB) : -6.41 2D Compatibility (Sec. Struct. Predict.) : 0.63 1D Compatibility (Hydrophobicity) : 0.64 QMean score : 0.327

(partial model without unconserved sides chains): PDB file : Tito_3F7W.pdb: (Unconserved sides chains are recalculated) : Sequence: align-3f7w-query.scw PDB file : Tito_Scwrl_3F7W.pdb: