Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MFKDIENRFGAKIQDIK--VLKSGWAGEIISLEFKDN------TQKYVIKTYNSSKN----GLENIKQEWTGLNLLYN-ANYPVPRPIISD-F-V-NEKPYIVMEKIEGENLWTCYQ---------TLSKEDRQQLLEKFVKVFLKLHELDVSI-IDKELRKDSTSSFIEKEINEIKKLVEENK----LEYF-TQIIDWLQKEKANI--IGEKLSIIHRDYHPWNVIVDNNKAIYVIDLLW-GIGDYRFDLAWMYTLMERSGF---------E---DFSQNALKKYKELKNQNINNFEYFKVFSTLRWLINVMISLKTGENLNETRNKEFENFVSPLIQNRIMSIKKITGIQITI 3ATT Chain:A ((22-318)) -------------VTVESGVDSTGMSSETIILTARWQQDGRSIQQKLVARVAPAAEDVPVFPTYRLDHQFEVIRLVGELTDVPVPRVRWIETTGDVLGTPFFLMDYVEGVVPPDVMPYTFGDNWFADAPAERQRQLQDATVAALATLHSIPNAQNTFSFLT----DTTLHRHFNWVRSWYDFAVEGIGRSPLLERTFEWLQSHW-PDDAAAREPVLLWGDARVGNVLYRDFQPVAVLDWEMVALGPRELDVAWMIFAHRVFQELAGLATLPGLPEVMREDDVRATYQALTGVELGDLHWFYVYSGVMWAC---------------------------------------------

General information: TITO was launched using: RESULT: Template: 3ATT.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1015 22161 21.83 90.09 target 2D structure prediction score : 0.56 Monomeric hydrophicity matching model chain A : 0.64 3D Compatibility (PKB) : 21.83 2D Compatibility (Sec. Struct. Predict.) : 0.56 1D Compatibility (Hydrophobicity) : 0.64 QMean score : 0.420

(partial model without unconserved sides chains): PDB file : Tito_3ATT.pdb: (Unconserved sides chains are recalculated) : Sequence: align-3att-query.scw PDB file : Tito_Scwrl_3ATT.pdb: