Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MLACGSDDLRTEEGLRIAALYGWDPEDTACQLLQ-RGENGTFLVR-TSGTRYILRRYRRERY-REQEI-LAELAWMCALR-DHINVPNVIANTEGDLATRLVGPDGERLYAVFEFIEGESPESS--SSEDYRRLGELMKALHRGADAIAQSMNEDWAGWNRPVYD-VQRAVREPLRHLLQF--EALS--EVNKTRCVSVARELERRFR---TLQPGRPFVHADLHFGNIVVRE---PNWYCLDFDECGYGHRAFDLGVVRLHLRN-----------RSDNFDCWAHFEHGYGD--MPSRGDLATGTAARIFYMAGKIPMRQDIDSLRHNPDARIGKYLTWIEDELM 4OCV Chain:A ((24-322)) ---------TNEALFDVASHFALEGTVDSIEPYGDGHINTTYLVTTD-GPRYILQRMNTGIFPDTVNLMRNVELVTSTLKAQGKETLDIVRTTSGDTWAEI----DGGAWRVYKFIEHTMSYNLVPNPDVFREAGRAFGDFQNFLSGFD--ANQLT--ETIAHFHDTPHRFEDFKKALAADELGRAAGCGPEIEFYLSHADQYAVVMDGLRDGSIPLRVTHNDTKLNNILMDATTGKARAIIDLDTIMPGSMLFDFGDSIRFGASTALEDERDLDKVHFSTELFRAYTEGFVGELRDSITAREA-ELLPFSGNLLTME----------------------------

General information: TITO was launched using: RESULT: Template: 4OCV.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1116 -10968 -9.83 -40.93 target 2D structure prediction score : 0.44 Monomeric hydrophicity matching model chain A : 0.64 3D Compatibility (PKB) : -9.83 2D Compatibility (Sec. Struct. Predict.) : 0.44 1D Compatibility (Hydrophobicity) : 0.64 QMean score : 0.279

(partial model without unconserved sides chains): PDB file : Tito_4OCV.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4ocv-query.scw PDB file : Tito_Scwrl_4OCV.pdb: