Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MISNTPSFPASWNIEAAKRIADTPAGIVYELTRKD-GSLAIAKVLKKKV--LKDSLRGADFIAWRAGIGCVELLDQSDNILLMEHA-GTETLRDVLFRDGNDDATTEIAAEVLLRYHQPSEQSPPS----------------SLLTLPLYFESLFRKAEQDR---S-------DGVDSPFVEAARLAQTLIDQQRDIKPLHGDLHHENIMHSD-RGWIIIDPA-GLIGDAALDVANMFSNPLDRFD---LTRSEARIASMAAIFSRALQRD-----ERLLLQYAFAYGCLSATWHEEDGNAEERDNELAVAATIKAVLKHF 2QG7 Chain:D ((114-433)) -----------------QIINGG-TNILIKVKDMSKQAKYLIRLYGPKTDNREREKKISCILYNK-NIA-KKIYVFFTNGRIEEFMDGY-ALSRED---IKNPKFQKLIAKNLKLLHDIKLNENLYKELQVTQKVPGTRPSFLWNTIWKYFHLLNEERKKICSFDAKANILKLIDFDVLRDSIVEVESLCKRENSPIVLCHCDLLSSNIINTVGDSISFIDFEYSCPMERAYDIANHFNEYAGFNCDWDLTPSKEEEYHFIMHYLGTDDEELINQLIREIQPFYICSHINWGLWSLLQGMHS-------------------

General information: TITO was launched using: RESULT: Template: 2QG7.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain D - contact count / total energy / energy per contact / energy per residue : 914 12440 13.61 52.27 target 2D structure prediction score : 0.65 Monomeric hydrophicity matching model chain D : 0.62 3D Compatibility (PKB) : 13.61 2D Compatibility (Sec. Struct. Predict.) : 0.65 1D Compatibility (Hydrophobicity) : 0.62 QMean score : 0.257

(partial model without unconserved sides chains): PDB file : Tito_2QG7.pdb: (Unconserved sides chains are recalculated) : Sequence: align-2qg7-query.scw PDB file : Tito_Scwrl_2QG7.pdb: