Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MVRRLVPFNLYAKKGRF----VMASEALATQYVSENTTIPVPRVLDVIALPSGKGKFLLMTGVKGKEYGPTGVTLYKMAENQRAVFTETLRGWFDQLRCLRPPDDHTISGFMVTGVSSY-IIRHPETVDPFASQDEFHAQRFCQPWEPYDDALRAALEKRAKMRYKICFTHGDITPHNILVDDN-LRPCALVDWECAGWMPEYWEYTRALYIRERY---LGWKKVFTDIFPNYEAELTIYGSIITHDMPWYYSLNVVS 4H05 Chain:A ((36-248)) ------GRELFVKVAALGAGVGLLGEAERLVWLAE-VGIPVPRVVEGGGD--ERVAWLVTEAVPGRPAS---ARW---PREQRLDVAVALAGLARSLHALDWER-----CPFDRSLAVTVPQAARAVAEGSVDLED---LDEERKG-WSGERLLAELERTRPADEDLAVCHGDLCPDNVLLDPRTCEVTGLIDVGRVGRADRHSDLALVLRELAHEEDPWFGPECSAAFLREYGRGW---------------------

General information: TITO was launched using: RESULT: Template: 4H05.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 903 -18139 -20.09 -88.92 target 2D structure prediction score : 0.66 Monomeric hydrophicity matching model chain A : 0.66 3D Compatibility (PKB) : -20.09 2D Compatibility (Sec. Struct. Predict.) : 0.66 1D Compatibility (Hydrophobicity) : 0.66 QMean score : 0.288

(partial model without unconserved sides chains): PDB file : Tito_4H05.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4h05-query.scw PDB file : Tito_Scwrl_4H05.pdb: