Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MSTTPDLPKERVITQEEFDNAVKL-GGKFAPVFKVDEKTVVKTSD-----MTQLGEAAAMKMVREK--TTIPVPEDQNVYTDPTTGDVPIVMDFIEGDCLLD-VWDTYDNDQKQQIIEQLHSYFAQLRELKGSFIGSVDGKFCFDQVFDQDIGGHGPYEDEASFNEGLVKALKNTMISGWGDTVCDMVL-----AMKDHEIVFTHGDISPRNILVKD-CNIVSVLDWEYSGYYPEYWEYAKAFFRP--AWEKSWIKDRAVNKILKPYPLENAICQHVFSFGAW 5IQC Chain:A ((28-257)) -----------------VDSIEIIGSGYDSVAYLVNNEYIFKTKFSTNKKKGYAKEKAIYNFLNTNLETNVKIPNIEYSYIS--DELSILGYKEIKGTFLTPEIYSTMSEEEQNLLKRDIASFLRQMHGLDYTDISECTIDNK-----------QNVLEEYILLRETIYNDLT----DIEKDYIESFMERLNATTVFEGKKCLCHNDFSCNHLLLDGNNRLTGIIDFGDSGIIDEYCDFIYLLEDSEEEIG-----TNFGEDILRMYGN--------------

General information: TITO was launched using: RESULT: Template: 5IQC.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 814 28032 34.44 131.60 target 2D structure prediction score : 0.60 Monomeric hydrophicity matching model chain A : 0.69 3D Compatibility (PKB) : 34.44 2D Compatibility (Sec. Struct. Predict.) : 0.60 1D Compatibility (Hydrophobicity) : 0.69 QMean score : 0.283

(partial model without unconserved sides chains): PDB file : Tito_5IQC.pdb: (Unconserved sides chains are recalculated) : Sequence: align-5iqc-query.scw PDB file : Tito_Scwrl_5IQC.pdb: