Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MISNTPSFPASWNIEAAKRIADTPAGIVYELTRKD-GSLAIAKVLKKKV-----LKDSLRGADFIAWRAGIGCVELLDQSDNILLMEHA-GTETLRDVLFRDGNDDATTEIAAEVLLRYHQPSEQSPPS----------------SLLTLPLYFESLFRKAEQDR---S-------DGVDSPFVEAARLAQTLIDQQRDIKPLHGDLHHENIMHSD--RGWIIIDPA-GLIGDAALDVANMFSNPLDRFD---LTRSEARIASMAAIFSRALQRD-----ERLLLQYAFAYGCLSATWHEEDGNAEERDNELAVAATIKAVLKHF 2QG7 Chain:E ((114-433)) -----------------QIING-ITNILIKVKDMSKQAKYLIRLYGPKTDEIINREREKKISCILYNK-NIA-KKIYVFFTNGRIEEFMDGY-ALSRED---IKNPKFQKLIAKNLKLLHDIKLNENLYKELQVTQKVPGTRPSFLWNTIWKYFHLLNEERKKICSFDAKANILKLIDFDVLRDSIVEVESLCKRENSPIVLCHCDLLSSNIINTVGGDSISFIDFEYSCPMERAYDIANHFNEYAGFNCDWDLTPSKEEEYHFIMHYLGTDDEELINQLIREIQPFYICSHINWGLWSLLQGMHS-------------------

General information: TITO was launched using: RESULT: Template: 2QG7.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain E - contact count / total energy / energy per contact / energy per residue : 911 1331 1.46 5.59 target 2D structure prediction score : 0.64 Monomeric hydrophicity matching model chain E : 0.61 3D Compatibility (PKB) : 1.46 2D Compatibility (Sec. Struct. Predict.) : 0.64 1D Compatibility (Hydrophobicity) : 0.61 QMean score : 0.263

(partial model without unconserved sides chains): PDB file : Tito_2QG7.pdb: (Unconserved sides chains are recalculated) : Sequence: align-2qg7-query.scw PDB file : Tito_Scwrl_2QG7.pdb: