Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MLACGSDDLRTEEGLRIAALYGWDPEDTACQLLQRGENGTFL-VR-TSGTRYILRRYRRERYREQEILAELAWMCALR-DHINVPNVIANTEGDLATRLVGPDGERLYAVFEFIEGESPESS-------SSEDYRRLGELMKALHRGADAIAQSMNEDWAGWNRPVYDVQRAVREPLRHLLQFEALSEVNKTRCVSVARELERRFRT-LQPGRPFVHADLHFGNIVVREP-----NWYCLDFDECGYGHRAFDLGVVRLHLRNRSDNFDCWAHFEHGYGDMPSRGDLATGTAAR-IFYMAGKIPMRQDIDSLRHNPDARIGKYLTWIEDELM 3CSV Chain:A ((4-276)) --------SREDEIRDFLATHGYA--DWNRTP-------RYQRLRSPTGAKAVLMDWSPEE--GGDTQPFVDLAQYLRNLDISAPEIYAEEH------------ARGLLLIEDLGDALFTEVINNDPAQEMPLYRAAVDLLIHLHDAQTPEL--AR-----LDPETLS--EMTRLAFSEYRY-AILGDAAEDNRKRFEHRFAQILSAQLEGDMVFVHRDFHAQNLLWLPEREGLARVGVIDFQDAKLGHRAYDLVSLLQDARR-DVPAQVEAQMIDHYIQATGVDESHF-RSAYAVIAVQRNMRILGI------------------------

General information: TITO was launched using: RESULT: Template: 3CSV.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1045 11341 10.85 45.73 target 2D structure prediction score : 0.40 Monomeric hydrophicity matching model chain A : 0.67 3D Compatibility (PKB) : 10.85 2D Compatibility (Sec. Struct. Predict.) : 0.40 1D Compatibility (Hydrophobicity) : 0.67 QMean score : 0.238

(partial model without unconserved sides chains): PDB file : Tito_3CSV.pdb: (Unconserved sides chains are recalculated) : Sequence: align-3csv-query.scw PDB file : Tito_Scwrl_3CSV.pdb: